ArticleThe EMBO journal2005
Dynamics of initiation, termination and reinitiation of DNA translocation by the motor protein EcoR124I.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed.
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation.Nucleic acids research · 2024Article
- Structural insights into assembly, operation and inhibition of a type I restriction-modification system.Nature microbiology · 2020Article
- Real-time detection of condensin-driven DNA compaction reveals a multistep binding mechanism.The EMBO journal · 2017Article
- CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases.Nucleic acids research · 2017Article
- Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes.Nature chemical biology · 2015Article
- ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation.Nucleic acids research · 2014Article
- Single molecule techniques in DNA repair: a primer.DNA repair · 2014Review
- Type I restriction enzymes and their relatives.Nucleic acids research · 2014Review
- Fork sensing and strand switching control antagonistic activities of RecQ helicases.Nature communications · 2013Article
- The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.Nucleic acids research · 2013Article
- Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes.Nucleic acids research · 2011Article
- An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme.Nucleic acids research · 2011Article
- Emerging area: biomaterials that mimic and exploit protein motion.Soft matter · 2011Article
- Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems.DNA research : an international journal for rapid publication of reports on genes and genomes · 2010Review
- Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Cleavage of a model DNA replication fork by a Type I restriction endonuclease.Nucleic acids research · 2009Article
- Type I restriction endonucleases are true catalytic enzymes.Nucleic acids research · 2009Article
- The structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein.Nucleic acids research · 2009Article
- The interrelationship of helicase and nuclease domains during DNA translocation by the molecular motor EcoR124I.Journal of molecular biology · 2008Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Type I restriction enzymes use two motors to translocate DNA before carrying out DNA cleavage. The motor function is accomplished by amino-acid motifs typical for superfamily 2 helicases, although DNA unwinding is not observed. Using a combination of extensive single-molecule magnetic tweezers and stopped-flow bulk measurements, we fully characterized the (re)initiation of DNA translocation by EcoR124I. We found that the methyltransferase core unit of the enzyme loads the motor subunits onto adjacent DNA by allowing them to bind and initiate translocation. Termination of translocation occurs owing to dissociation of the motors from the core unit. Reinitiation of translocation requires binding of new motors from solution. The identification and quantification of further initiation steps--ATP binding and extrusion of an initial DNA loop--allowed us to deduce a complete kinetic reinitiation scheme. The dissociation/reassociation of motors during translocation allows dynamic control of the restriction process by the availability of motors. Direct evidence that this control mechanism is relevant in vivo is provided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.