ArticleMolecular biology of the cell2006
RhoA GTPase regulates M-cadherin activity and myoblast fusion.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 129 citations in OpenAlex.
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- Developmental dynamics of skeletal muscle can be recapitulated in vitro from pig embryonic stem cells.Stem cell reports · 2026Article
- Planar cell polarity protein Vangl2 interacts with M-cadherin and stabilizes its cell surface expression in mouse C2C12 myoblasts.Frontiers in cell and developmental biology · 2026Article
- Temporal transcriptomic profiling of human three-dimensional neuromuscular co-cultures.Biology open · 2025Article
- GRAF1 deficiency leads to defective brown adipose tissue differentiation and thermogenic response.Scientific reports · 2024Article
- Substrate stress relaxation regulates neural stem cell fate commitment.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function.International journal of molecular sciences · 2024Article
- RhoA Is a Crucial Regulator of Myoblast Fusion.Cells · 2023Article
- Review
- Lamina-associated polypeptide 2α is required for intranuclear MRTF-A activity.Scientific reports · 2022Article
- Syndecan-4 affects myogenesis via Rac1-mediated actin remodeling and exhibits copy-number amplification and increased expression in human rhabdomyosarcoma tumors.Cellular and molecular life sciences : CMLS · 2022Article
- Role of RHO family interacting cell polarization regulators (RIPORs) in health and disease: Recent advances and prospects.International journal of biological sciences · 2022Review
- Article
- Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis.Skeletal muscle · 2021Article
- A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1-RhoA/Rac1.Journal of cellular and molecular medicine · 2021Article
- Article
- Article
- Post-transcriptional regulation of MRTF-A by miRNAs during myogenic differentiation of myoblasts.Nucleic acids research · 2020Article
- The regulatory role of Myomaker and Myomixer-Myomerger-Minion in muscle development and regeneration.Cellular and molecular life sciences : CMLS · 2020Review
- Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rho family of GTP-binding proteins plays critical roles during myogenesis induction. To elucidate their role later during myogenesis, we have analyzed RhoA function during myoblast fusion into myotubes. We find that RhoA activity is rapidly and transiently increased when cells are shifted into differentiation medium and then is decreased until myoblast fusion. RhoA activity must be down-regulated to allow fusion, because expression of a constitutively active form of RhoA (RhoAV14) inhibits this process. RhoAV14 perturbs the expression and localization of M-cadherin, a member of the Ca2+-dependent cell-cell adhesion molecule family that has an essential role in skeletal muscle cell differentiation. This mutant does not affect N-cadherin and other proteins involved in myoblast fusion, beta1-integrin and ADAM12. Active RhoA induces the entry of M-cadherin into a degradative pathway and thus decreases its stability in correlation with the monoubiquitination of M-cadherin. Moreover, p120 catenin association with M-cadherin is decreased in RhoAV14-expressing cells, which is partially reverted by the inhibition of the RhoA effector Rho-associated kinase ROCK. ROCK inhibition also restores M-cadherin accumulation at the cell-cell contact sites. We propose that the sustained activation of the RhoA pathway inhibits myoblast fusion through the regulation of p120 activity, which controls cadherin internalization and degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.