ArticleJournal of bacteriology2005
Characterization of temporal protein production in Pseudomonas aeruginosa biofilms.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
111 citing papers in PubMed, 218 citations in OpenAlex.
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- Exploring the anti-biofilm effect of darobactin B and colistin in static and dynamic environments.Microbiology spectrum · 2026Article
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- Exploration of Phytochemicals as Anti-biofilm Agents against Pathogenic Bacteria: Their Potential and Challenges.Infectious disorders drug targets · 2025Review
- Staphyloccocus aureus biofilm, in absence of planktonic bacteria, produces factors that activate counterbalancing inflammatory and immune-suppressive genes in human monocytes.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Biofilm formation ofHeliyon · 2024Article
- Emerging implications of bacterial biofilm in cancer biology: Recent updates and major perspectives.Gut microbes reports · 2024Review
- Untargeted metabolomics unveiled the role of butanoate metabolism in the development ofFrontiers in cellular and infection microbiology · 2024Article
- A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis.Biomedical microdevices · 2023Article
- Effect of cetrimonium carrier micelles on bacterial membranes and extracellular DNA, an in silico study.Scientific reports · 2023Article
- Toxin-linked mobile genetic elements in major enteric bacterial pathogens.Gut microbiome (Cambridge, England) · 2023Review
- Formation of unique T-shape budding and differential impacts of low surface water on Bacillus mycoides rhizoidal colony.Archives of microbiology · 2022Article
- Hurdle technology using encapsulated enzymes and essential oils to fight bacterial biofilms.Applied microbiology and biotechnology · 2022Review
- The Two-Component System FleS/FleR Represses H1-T6SS via Cyclic di-GMP Signaling in Pseudomonas aeruginosa.Applied and environmental microbiology · 2022Article
- Imaging flow cytometry reveals a dual role for exopolysaccharides in biofilms: To promote self-adhesion while repelling non-self-community members.Computational and structural biotechnology journal · 2022Article
- Alternatives to Conventional Antibiotic Therapy: Potential Therapeutic Strategies of Combating Antimicrobial-Resistance and Biofilm-Related Infections.Molecular biotechnology · 2021Review
- Three faces of biofilms: a microbial lifestyle, a nascent multicellular organism, and an incubator for diversity.NPJ biofilms and microbiomes · 2021Review
- Genetic analysis of electroactive biofilms.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- New roles for glutathione: Modulators of bacterial virulence and pathogenesis.Redox biology · 2021Article
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Phenotypic and genetic evidence supporting the notion of biofilm formation as a developmental process is growing. In the present work, we provide additional support for this hypothesis by identifying the onset of accumulation of biofilm-stage specific proteins during Pseudomonas aeruginosa biofilm maturation and by tracking the abundance of these proteins in planktonic and three biofilm developmental stages. The onset of protein production was found to correlate with the progression of biofilms in developmental stages. Protein identification revealed that proteins with similar function grouped within similar protein abundance patterns. Metabolic and housekeeping proteins were found to group within a pattern separate from virulence, antibiotic resistance, and quorum-sensing-related proteins. The latter were produced in a progressive manner, indicating that attendant features that are characteristic of biofilms such as antibiotic resistance and virulence may be part of the biofilm developmental process. Mutations in genes for selected proteins from several protein production patterns were made, and the impact of these mutations on biofilm development was evaluated. The proteins cytochrome c oxidase, a probable chemotaxis transducer, a two-component response regulator, and MexH were produced only in mature and late-stage biofilms. Mutations in the genes encoding these proteins did not confer defects in growth, initial attachment, early biofilm formation, or twitching motility but were observed to arrest biofilm development at the stage of cell cluster formation we call the maturation-1 stage. The results indicated that expression of theses genes was required for the progression of biofilms into three-dimensional structures on abiotic surfaces and the completion of the biofilm developmental cycle. Reverse transcription-PCR analysis confirmed the detectable change in expression of the respective genes ccoO, PA4101, and PA4208. We propose a possible mechanism for the role of these biofilm-specific proteins in biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.