ArticleJournal of bacteriology2005
Increased excision of the Salmonella prophage ST64B caused by a deficiency in Dam methylase.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- In silico design and evaluation of a candidate multiepitope Salmonella vaccine construct targeting broad-spectrum protection in poultry.BMC genomics · 2026Article
- Reduced ROS-associated prophage induction in a lepA mutant contributes to increased fluoroquinolone persistence in Salmonella Typhimurium.Scientific reports · 2026Article
- Dam-dependent epigenetic memory regulates prophage reintegration in Salmonella.Nucleic acids research · 2025Article
- TisB enables antibiotic tolerance in Salmonella by preventing prophage induction through ATP depletion.PLoS pathogens · 2025Article
- Prophage Gifsy-1 Induction in Salmonella enterica Serovar Typhimurium Reduces Persister Cell Formation after Ciprofloxacin Exposure.Microbiology spectrum · 2023Article
- The bacterial epigenome.Nature reviews. Microbiology · 2020Review
- Prophages in Salmonella enterica: a driving force in reshaping the genome and physiology of their bacterial host?Molecular microbiology · 2019Review
- DNA Methylation.EcoSal Plus · 2014Article
- Transcriptional Profiling of a Cross-ProtectivePathogens (Basel, Switzerland) · 2014Article
- Escherichia coli DNA adenine methyltransferase: the structural basis of processive catalysis and indirect read-out.The Journal of biological chemistry · 2009Article
- Roles of DNA adenine methylation in host-pathogen interactions: mismatch repair, transcriptional regulation, and more.FEMS microbiology reviews · 2009Review
- Regulation of the Salmonella enterica std fimbrial operon by DNA adenine methylation, SeqA, and HdfR.Journal of bacteriology · 2008Article
- Modulation of Escherichia coli DNA methyltransferase activity by biologically derived GATC-flanking sequences.The Journal of biological chemistry · 2008Article
- Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7.Journal of bacteriology · 2008Article
- DNA adenine methylation regulates virulence gene expression in Salmonella enterica serovar Typhimurium.Journal of bacteriology · 2006Article
- Epigenetic gene regulation in the bacterial world.Microbiology and molecular biology reviews : MMBR · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica mutants defective in Dam methylase are strongly attenuated in virulence and release a large amount of proteins to the extracellular medium. The extent to which these two phenotypes are linked is unknown. Using a proteomic approach, we identified Sb6, Sb13, and Sb36 as proteins present in larger amounts in culture supernatants of an S. enterica serovar Typhimurium dam mutant than in those of the wild-type strain. These three proteins are encoded in the Salmonella prophage ST64B. Higher amounts of ST64B phage DNA and tailless viral capsids were also detected in supernatant extracts of the dam mutant, suggesting that Dam methylation negatively regulates the excision of ST64B. Reverse transcription-PCR analysis revealed that the expression of two ST64B genes encoding a putative antirepressor and a phage replication protein increases in the dam mutant. The SOS response also augments the excision of ST64B. Infection assays performed with phage-cured strains demonstrated that ST64B does not carry genes required for virulence in the mouse model. Evidence was also obtained discarding a relationship between the high excision of ST64B and the envelope instability or virulence attenuation phenotype. Taken together, these data indicate that ST64B excises at a high rate in dam mutants due to the loss of repression exerted by Dam on phage genes and induction of the SOS response characteristic of these mutants. The exacerbated excision of ST64B does not however contribute to the incapacity of dam mutants to cause disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.