ArticleThe Biochemical journal2006
A Toxoplasma gondii phosphoinositide phospholipase C (TgPI-PLC) with high affinity for phosphatidylinositol.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Functional analysis of CaParasitology · 2026Article
- Regulation of phosphoinositide metabolism in Apicomplexan parasites.Frontiers in cell and developmental biology · 2023Review
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- A positive feedback loop mediates crosstalk between calcium, cyclic nucleotide and lipid signalling in calcium-induced Toxoplasma gondii egress.PLoS pathogens · 2022Article
- Global proteomic profiling of multiple organs of cats (Felis catus) and proteome-transcriptome correlation during acute Toxoplasma gondii infection.Infectious diseases of poverty · 2022Article
- Temporal and thermal profiling of theeLife · 2022Article
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- Calcium signaling in intracellular protist parasites.Current opinion in microbiology · 2021Review
- Article
- Diverse Roles of TgMIC1/4/6 in theFrontiers in microbiology · 2021Review
- The effect of edelfosine on GRA1 and MIC3 expressions in acute toxoplasmosis.Parasitology research · 2020Article
- Centrin2 from the human parasiteJournal of cell science · 2019Article
- Characterization of Two EF-hand Domain-containing Proteins from Toxoplasma gondii.The Journal of eukaryotic microbiology · 2019Article
- Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii.Biochimica et biophysica acta. Molecular cell research · 2018Review
- Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites.Nature communications · 2018Article
- Tagging of Weakly Expressed Toxoplasma gondii Calcium-Related Genes with High-Affinity Tags.The Journal of eukaryotic microbiology · 2018Article
- InsP3 Signaling in Apicomplexan Parasites.Current topics in medicinal chemistry · 2017Review
- Calcium Signaling throughout the Toxoplasma gondii Lytic Cycle: A STUDY USING GENETICALLY ENCODED CALCIUM INDICATORS.The Journal of biological chemistry · 2015Article
- The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp.Cell calcium · 2015Review
- Intracellular calcium channels in protozoa.European journal of pharmacology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Toxoplasma gondii phosphoinositide-specific phospholipase C gene (TgPI-PLC) was cloned, sequenced and expressed in Escherichia coli and its enzymatic characteristics were investigated. TgPI-PLC is present in the genome as a single-copy gene consisting of 22 exons interrupted by 21 introns, and encodes a polypeptide of 1097 amino acids with a predicted molecular mass of 121 kDa. In addition to the conserved catalytic X and Y domains, TgPI-PLC contains an apparent N-terminal PH domain, an EF hand motif and a C-terminal C2 domain. When compared with mammalian delta-type PI-PLC, TgPI-PLC has an additional extended N-terminus and two insertions in the region between the X and Y domains, with a 31-35% identity over the whole sequence. Recombinant TgPI-PLC, as well as the native enzyme obtained from crude membrane extracts of the parasite, was more active with phosphatidylinositol than with phosphatidylinositol 4,5-bisphosphate as substrate. Indirect immunofluorescence analysis using an affinity-purified antibody against TgPI-PLC revealed that this enzyme localizes in the plasma membrane of the parasites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.