ArticleMolecular and cellular biology2005
Multisite M-phase phosphorylation of Xenopus Wee1A.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells.bioRxiv : the preprint server for biology · 2026Article
- ERK synchronizes embryonic cleavages in Drosophila.Developmental cell · 2024Article
- Combined evaluation of both WEE1 and phosphorylated cyclin dependent kinase 1 expressions in oral squamous cell carcinomas predicts cancer recurrence and progression.Journal of dental sciences · 2022Article
- Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators.Molecular biology of the cell · 2022Article
- Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate Phosphorylation.Current biology : CB · 2021Article
- Wee1 and Cdc25 are controlled by conserved PP2A-dependent mechanisms in fission yeast.Cell cycle (Georgetown, Tex.) · 2017Article
- Deciphering the Interplay among Multisite Phosphorylation, Interaction Dynamics, and Conformational Transitions in a Tripartite Protein System.ACS central science · 2016Article
- The Prozone Effect Accounts for the Paradoxical Function of the Cdk-Binding Protein Suc1/Cks.Cell reports · 2016Article
- Cdk1 plays matchmaker for the Polo-like kinase and its activator SPAT-1/Bora.Cell cycle (Georgetown, Tex.) · 2015Article
- Unravelling the pivotal role of Alix in MVB sorting and silencing of the activated EGFR.The Biochemical journal · 2015Article
- The Cdk1-APC/C cell cycle oscillator circuit functions as a time-delayed, ultrasensitive switch.Nature cell biology · 2013Article
- A cell-free system for functional centromere and kinetochore assembly.Nature protocols · 2012Article
- Short-term integration of Cdc25 dynamics controls mitotic entry during Drosophila gastrulation.Developmental cell · 2012Article
- Morphogenesis and the cell cycle.Genetics · 2012Review
- A phosphatase threshold sets the level of Cdk1 activity in early mitosis in budding yeast.Molecular biology of the cell · 2011Article
- Article
- Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited.Molecular biology of the cell · 2011Article
- Ultrasensitivity in the Regulation of Cdc25C by Cdk1.Molecular cell · 2011Article
- Multisite phosphorylation provides an effective and flexible mechanism for switch-like protein degradation.PloS one · 2010Article
- Nonessential sites improve phosphorylation switch.Biophysical journal · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Cdk1 inhibitor Wee1 is inactivated during mitotic entry by proteolysis, translational regulation, and transcriptional regulation. Wee1 is also regulated by posttranslational modifications, and here we have identified five phosphorylation sites in the N-terminal domain of embryonic Xenopus Wee1A through a combination of mutagenesis studies and matrix-assisted laser desorption ionization-time of flight mass spectrometry. All five sites conform to the Ser-Pro/Thr-Pro consensus for proline-directed kinases like Cdks. Three of the sites (Ser 38, Thr 53, and Ser 62) are required for the mitotic gel shift, and at least two of these sites (Ser 38 and Thr 53) regulate the proteolysis of Wee1A during interphase. The other two sites (Thr 104 and Thr 150) are primarily responsible for the mitotic inactivation of Wee1A. Alanine mutants of Thr 150 or Thr 104 had an increased capacity to inhibit mitotic entry in cyclin B-treated interphase extracts, and Thr 150 was found to be transiently phosphorylated just prior to nuclear envelope breakdown in cycling egg extracts. These findings establish the phosphorylation-dependent direct inactivation of Wee1A as a critical mechanism for the promotion of M-phase entry. These results also show that multisite phosphorylation cooperatively inactivates Wee1A and cooperatively promotes Wee1A proteolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.