ArticleMolecular and cellular biology2005
Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- NFE2 Truncation Mutants Protect Wild-Type NFE2 from ITCH-Dependent Degradation.International journal of molecular sciences · 2025Article
- Prolonged fasting and glucocorticoid exposure drive dynamic DNA methylation in northern elephant seals.The Journal of experimental biology · 2025Article
- Concomitant constitutive LNK and NFE2 mutation with loss of sumoylation in a case of hereditary thrombocythemia.Haematologica · 2021Article
- SUMO and Transcriptional Regulation: The Lessons of Large-Scale Proteomic, Modifomic and Genomic Studies.Molecules (Basel, Switzerland) · 2021Review
- NF-E2 mutation as a novel cause for inherited thrombocytopenia.British journal of haematology · 2020Article
- miR-122-mediated translational repression of PEG10 and its suppression in human hepatocellular carcinoma.Journal of translational medicine · 2016Article
- Regulation and function of the NFE2 transcription factor in hematopoietic and non-hematopoietic cells.Cellular and molecular life sciences : CMLS · 2015Review
- Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes.Nucleic acids research · 2012Article
- Article
- SUMOylation regulates the transcriptional repression activity of FOG-2 and its association with GATA-4.PloS one · 2012Article
- Sumoylation regulates interaction of FOG1 with C-terminal-binding protein (CTBP).The Journal of biological chemistry · 2010Article
- Developmental silencing of human zeta-globin gene expression is mediated by the transcriptional repressor RREB1.The Journal of biological chemistry · 2010Article
- JNK-mediated turnover and stabilization of the transcription factor p45/NF-E2 during differentiation of murine erythroleukemia cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Extracellular signal-regulated kinase mitogen-activated protein kinase signaling initiates a dynamic interplay between sumoylation and ubiquitination to regulate the activity of the transcriptional activator PEA3.Molecular and cellular biology · 2009Article
- Sumoylation of EKLF promotes transcriptional repression and is involved in inhibition of megakaryopoiesis.Molecular and cellular biology · 2007Article
- Review
- Systematic RNAi studies on the role of Sp/KLF factors in globin gene expression and erythroid differentiation.Journal of molecular biology · 2007Article
- Subcellular transport of EKLF and switch-on of murine adult beta maj globin gene transcription.Molecular and cellular biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells and in mouse fetal liver. By in vitro sumoylation reaction and DNA transfection experiments, we show that the sumoylation occurs at lysine 368 (K368) of human p45/NF-E2. Furthermore, p45 sumoylation enhances the transactivation capability of NF-E2, and this is accompanied by an increase of the NF-E2 DNA binding affinity. More interestingly, we have found that in K562 cells, the beta-globin gene loci in the euchromatin regions are predominantly colocalized with the nuclear bodies promyelocytic leukemia protein (PML) oncogenic domains that are enriched with the PML, SUMO-1, RNA polymerase II, and sumoylatable p45/NF-E2. Chromatin immunoprecipitation assays further showed that the intact sumoylation site of p45/NF-E2 is required for its binding to the DNase I-hypersensitive sites of the beta-globin locus control region. Finally, we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue beta-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian beta-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.