ArticleMolecular and cellular biology2005
Loss of SOD1 and LYS7 sensitizes Saccharomyces cerevisiae to hydroxyurea and DNA damage agents and downregulates MEC1 pathway effectors.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- High-throughput screening identifies a previously undescribed checkpoint controlling mitotic progression in response to DNA damage.The FEBS journal · 2025Article
- Mitochondrial superoxide dismutase Sod2 suppresses nuclear genome instability during oxidative stress.Genetics · 2023Article
- Nuclear SOD1 in Growth Control, Oxidative Stress Response, Amyotrophic Lateral Sclerosis, and Cancer.Antioxidants (Basel, Switzerland) · 2022Review
- Genetically induced redox stress occurs in a yeast model for Roberts syndrome.G3 (Bethesda, Md.) · 2022Article
- Fingolimod Potentiates the Antifungal Activity of Amphotericin B.Frontiers in cellular and infection microbiology · 2021Article
- Functional interplay between the oxidative stress response and DNA damage checkpoint signaling for genome maintenance in aerobic organisms.Journal of microbiology (Seoul, Korea) · 2020Review
- DNA damage induces Yap5-dependent transcription of ECO1/CTF7 in Saccharomyces cerevisiae.PloS one · 2020Article
- Synthetic lethal interaction between oxidative stress response and DNA damage repair in the budding yeast and its application to targeted anticancer therapy.Journal of microbiology (Seoul, Korea) · 2019Review
- Complementary function of two transketolase isoforms from Moniliella megachiliensis in relation to stress response.AMB Express · 2017Article
- Heme deficiency sensitizes yeast cells to oxidative stress induced by hydroxyurea.The Journal of biological chemistry · 2017Article
- Unraveling new functions of superoxide dismutase using yeast model system: Beyond its conventional role in superoxide radical scavenging.Journal of microbiology (Seoul, Korea) · 2017Review
- The Cell Killing Mechanisms of Hydroxyurea.Genes · 2016Review
- The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016Review
- Expanding roles of superoxide dismutases in cell regulation and cancer.Drug discovery today · 2016Review
- Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 in DNA replication initiation.Protein & cell · 2015Article
- Loss of APD1 in yeast confers hydroxyurea sensitivity suppressed by Yap1p transcription factor.Scientific reports · 2015Article
- DNA Damage Response Checkpoint Activation Drives KP1019 Dependent Pre-Anaphase Cell Cycle Delay in S. cerevisiae.PloS one · 2015Article
- Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance.Nature communications · 2014Article
- Cytoplasmic localization of Hug1p, a negative regulator of the MEC1 pathway, coincides with the compartmentalization of Rnr2p-Rnr4p.Biochemical and biophysical research communications · 2013Article
- The yeast copper response is regulated by DNA damage.Molecular and cellular biology · 2013Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Aerobic metabolism produces reactive oxygen species, including superoxide anions, which cause DNA damage unless removed by scavengers such as superoxide dismutases. We show that loss of the Cu,Zn-dependent superoxide dismutase, SOD1, or its copper chaperone, LYS7, confers oxygen-dependent sensitivity to replication arrest and DNA damage in Saccharomyces cerevisiae. We also find that sod1Delta strains, and to a lesser extent lys7Delta strains, when arrested with hydroxyurea (HU) show reduced induction of the MEC1 pathway effector Rnr3p and of Hug1p. The HU sensitivity of sod1Delta and lys7Delta strains is suppressed by overexpression of TKL1, a transketolase that generates NADPH, which balances redox in the cell and is required for ribonucleotide reductase activity. Our results suggest that the MEC1 pathway in sod1Delta mutant strains is sensitive to the altered cellular redox state due to increased superoxide anions and establish a new relationship between SOD1, LYS7, and the MEC1-mediated checkpoint response to replication arrest and DNA damage in S. cerevisiae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.