ArticleMolecular and cellular biology2005
The iStem, a long-range RNA secondary structure element required for efficient exon inclusion in the Drosophila Dscam pre-mRNA.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Current insights into insect immune memory.eLife · 2025Review
- Plasmid-based gap-repair recombineered transgenes reveal a central role for introns in mutually exclusive alternative splicing in Down Syndrome Cell Adhesion Molecule exon 4.Nucleic acids research · 2019Article
- RNA structure in splicing: An evolutionary perspective.RNA biology · 2016Review
- A large family of Dscam genes with tandemly arrayed 5' cassettes in Chelicerata.Nature communications · 2016Article
- RNA structure replaces the need for U2AF2 in splicing.Genome research · 2016Article
- IRBIS: a systematic search for conserved complementarity.RNA (New York, N.Y.) · 2014Article
- Rbfox proteins regulate alternative mRNA splicing through evolutionarily conserved RNA bridges.Nature structural & molecular biology · 2013Article
- Competition between pre-mRNAs for the splicing machinery drives global regulation of splicing.Molecular cell · 2013Article
- Ultra-deep profiling of alternatively spliced Drosophila Dscam isoforms by circularization-assisted multi-segment sequencing.The EMBO journal · 2013Article
- Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.PeerJ · 2013Article
- An RNA architectural locus control region involved in Dscam mutually exclusive splicing.Nature communications · 2012Article
- Mirtrons: microRNA biogenesis via splicing.Biochimie · 2011Review
- RNA structure and the mechanisms of alternative splicing.Current opinion in genetics & development · 2011Review
- Predicting in vivo binding sites of RNA-binding proteins using mRNA secondary structure.RNA (New York, N.Y.) · 2010Article
- Role of RNA structure in regulating pre-mRNA splicing.Trends in biochemical sciences · 2010Review
- Massive expansions of Dscam splicing diversity via staggered homologous recombination during arthropod evolution.RNA (New York, N.Y.) · 2010Article
- Modulation of alternative splicing by long-range RNA structures in Drosophila.Nucleic acids research · 2009Article
- Multiple and specific mRNA processing targets for the major human hnRNP proteins.Molecular and cellular biology · 2008Article
- Conserved secondary structures in Aspergillus.PloS one · 2008Article
- "Nought may endure but mutability": spliceosome dynamics and the regulation of splicing.Molecular cell · 2008Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila Dscam gene encodes 38,016 different proteins, due to alternative splicing of 95 of its 115 exons, that function in axon guidance and innate immunity. The alternative exons are organized into four clusters, and the exons within each cluster are spliced in a mutually exclusive manner. Here we describe an evolutionarily conserved RNA secondary structure we call the Inclusion Stem (iStem) that is required for efficient inclusion of all 12 variable exons in the exon 4 cluster. Although the iStem governs inclusion or exclusion of the entire exon 4 cluster, it does not play a significant role in determining which variable exon is selected. Thus, the iStem is a novel type of regulatory element that simultaneously controls the splicing of multiple alternative exons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.