ArticleBMC microbiology2005
Evaluation and selection of tandem repeat loci for Streptococcus pneumoniae MLVA strain typing.
Article in BMC microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular Epidemiology ofInternational journal of molecular sciences · 2025Pooled it
- Comparative Genotyping of Malaysian Clinical Isolates ofThe Malaysian journal of medical sciences : MJMS · 2025Article
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- Diversification and Distribution of Ruminant Chlamydia abortus Clones Assessed by MLST and MLVA.PloS one · 2015Article
- Phenotypic and molecular characterization of optochin-resistant Streptococcus pneumoniae isolates from Brazil, with description of five novel mutations in the ATPC gene.Journal of clinical microbiology · 2013Article
- Software for selecting the most informative sets of genomic loci for multi-target microbial typing.BMC bioinformatics · 2013Article
- Multiple-locus variable-number tandem-repeat analysis of Streptococcus pneumoniae and comparison with multiple loci sequence typing.BMC microbiology · 2012Article
- Population structure of hyperinvasive serotype 12F, clonal complex 218 Streptococcus pneumoniae revealed by multilocus boxB sequence typing.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Article
- A multi locus variable number of tandem repeat analysis (MLVA) scheme for Streptococcus agalactiae genotyping.BMC microbiology · 2011Article
- Multilocus variable number tandem repeat analysis for Salmonella enterica subspecies.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Review
- Identification of variable-number tandem-repeat (VNTR) sequences in Acinetobacter baumannii and interlaboratory validation of an optimized multiple-locus VNTR analysis typing scheme.Journal of clinical microbiology · 2011Article
- Multiple-locus variable number tandem repeat analysis for Streptococcus pneumoniae: comparison with PFGE and MLST.PloS one · 2011Article
- Expansion and evolution of the Streptococcus pneumoniae Spain9V-ST156 clonal complex in Poland.Antimicrobial agents and chemotherapy · 2010Article
- Molecular typing of pneumococci for investigation of linked cases of invasive pneumococcal disease.Journal of clinical microbiology · 2010Article
- PCR-based rapid genotyping of Stenotrophomonas maltophilia isolates.BMC microbiology · 2008Article
- Evaluation and strategy for use of MIRU-VNTRplus, a multifunctional database for online analysis of genotyping data and phylogenetic identification of Mycobacterium tuberculosis complex isolates.Journal of clinical microbiology · 2008Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrecise identification of bacterial pathogens at the strain level is essential for epidemiological purposes. In Streptococcus pneumoniae, the existence of 90 different serotypes makes the typing particularly difficult and requires the use of highly informative tools. Available methods are relatively expensive and cannot be used for large-scale or routine typing of any new isolate. We explore here the potential of MLVA (Multiple Loci VNTR Analysis; VNTR, Variable Number of Tandem Repeats), a method of growing importance in the field of molecular epidemiology, for genotyping of Streptococcus pneumoniae.
resultsAvailable genome sequences were searched for polymorphic tandem repeats. The loci identified were typed across a collection of 56 diverse isolates and including a group of serotype 1 isolates from Africa. Eventually a set of 16 VNTRs was proposed for MLVA-typing of S. pneumoniae. These robust markers were sufficient to discriminate 49 genotypes and to aggregate strains on the basis of the serotype and geographical origin, although some exceptions were found. Such exceptions may reflect serotype switching or horizontal transfer of genetic material.
conclusionWe describe a simple PCR-based MLVA genotyping scheme for S. pneumoniae which may prove to be a powerful complement to existing tools for epidemiological studies. Using this technique we uncovered a clonal population of strains, responsible for infections in Burkina Faso. We believe that the proposed MLVA typing scheme can become a standard for epidemiological studies of S. pneumoniae.
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