ArticlePlant methods2005
Blue-native PAGE in plants: a tool in analysis of protein-protein interactions.
Article in Plant methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
68 citing papers in PubMed.
- DNase Activities of a Highly Stable High-Molecular-Mass Multiprotein Complex Isolated from Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- Phosphatase Activities of a Highly Stable High-Molecular-Mass Multiprotein Complex Isolated from Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- Stable and Specific Multiprotein Complexes with High Molecular Masses in Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.Plant cell reports · 2026Article
- Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Very Stable High-Molecular-Mass Multiprotein Complexes in Different Organs of the Sea CucumberMolecules (Basel, Switzerland) · 2025Article
- The complex I subunit B22 contains a LYR domain that is crucial for an interaction with the mitochondrial acyl carrier protein SDAP1.The Plant journal : for cell and molecular biology · 2025Article
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST.The Plant cell · 2023Article
- Controlling the separation of native proteins with temperature in thermal gel transient isotachophoresis.Analytical and bioanalytical chemistry · 2023Article
- Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis.Plant physiology · 2023Article
- Effector-dependent activation and oligomerization of plant NRC class helper NLRs by sensor NLR immune receptors Rpi-amr3 and Rpi-amr1.The EMBO journal · 2023Article
- Combining BN-PAGE and microscopy techniques to investigate pigment-protein complexes and plastid transitions in citrus fruit.Plant methods · 2022Article
- Mitochondrial activity and biogenesis during resurrection of Haberlea rhodopensis.The New phytologist · 2022Article
- Review
- Protease and DNase Activities of a Very Stable High-Molecular-Mass Multiprotein Complex from Sea CucumberInternational journal of molecular sciences · 2022Article
- The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis.The Plant journal : for cell and molecular biology · 2022Article
- Comparison of the Content of Several Elements in Seawater, Sea CucumberMolecules (Basel, Switzerland) · 2022Article
- Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE.Plant methods · 2022Article
- Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea CucumberMolecules (Basel, Switzerland) · 2021Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.