ArticleMolecular imaging
Micro-PET/CT monitoring of herpes thymidine kinase suicide gene therapy in a prostate cancer xenograft: the advantage of a cell-specific transcriptional targeting approach.
Article in Molecular imaging. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Canny edge-based deformable image registration.Physics in medicine and biology · 2017Article
- A molecular imaging system based on both transcriptional and genomic amplification to detect prostate cancer cells in vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Molecular imaging of prostate cancer: PET radiotracers.AJR. American journal of roentgenology · 2012Review
- Androgen-independent molecular imaging vectors to detect castration-resistant and metastatic prostate cancer.Cancer research · 2011Article
- Experimental models to study lymphatic and blood vascular metastasis.Journal of surgical oncology · 2011Review
- In vivo imaging of intraprostatic-specific gene transcription by PET.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2011Article
- Magnetic resonance imaging of cells in experimental disease models.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Non-invasive molecular imaging of prostate cancer lymph node metastasis.Trends in molecular medicine · 2009Review
- Titration of variant HSV1-tk gene expression to determine the sensitivity of 18F-FHBG PET imaging in a prostate tumor.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2009Article
- A potent, imaging adenoviral vector driven by the cancer-selective mucin-1 promoter that targets breast cancer metastasis.Clinical cancer research : an official journal of the American Association for Cancer Research · 2009Article
- Imaging of viral thymidine kinase gene expression by replicating oncolytic adenovirus and prediction of therapeutic efficacy.Yonsei medical journal · 2008Article
- Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer.Nature medicine · 2008Article
- Configurations of a two-tiered amplified gene expression system in adenoviral vectors designed to improve the specificity of in vivo prostate cancer imaging.Gene therapy · 2008Article
- Advances in preclinical investigation of prostate cancer gene therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2007Review
- Specific targeting of gene therapy to prostate cancer using a two-step transcriptional amplification system.Future oncology (London, England) · 2006Review
- Molecular imaging of prostate cancer: a concise synopsis.Molecular imagingReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Cancer gene therapy based on tissue-restricted expression of cytotoxic gene should achieve superior therapeutic index over an unrestricted method. This study compared the therapeutic effects of a highly augmented, prostate-specific gene expression method to a strong constitutive promoter-driven approach. Molecular imaging was coupled to gene therapy to ascertain real-time therapeutic activity. The imaging reporter gene (luciferase) and the cytotoxic gene (herpes simplex thymidine kinase) were delivered by adenoviral vectors injected directly into human prostate tumors grafted in SCID mice. Serial bioluminescence imaging, positron emission tomography, and computed tomography revealed restriction of gene expression to the tumors when prostate-specific vector was employed. In contrast, administration of constitutive active vector resulted in strong signals in the liver. Liver serology, tissue histology, and frail condition of animals confirmed liver toxicity suffered by the constitutive active cohorts, whereas the prostate-targeted group was unaffected. The extent of tumor killing was analyzed by apoptotic staining and human prostate marker (prostate-specific antigen). Overall, the augmented prostate-specific expression system was superior to the constitutive approach in safeguarding against systemic toxicity, while achieving effective tumor killing. Integrating noninvasive imaging into cytotoxic gene therapy will provide a useful strategy to monitor gene expression and therapeutic efficacy in future clinical protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.