ArticleThe Journal of physiology2006
An amino-terminal lysine residue of rat connexin40 that is required for spermine block.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- The Polyamine Spermine Potentiates the Propagation of Negatively Charged Molecules through the Astrocytic Syncytium.Biomolecules · 2022Article
- Polyamines regulate phosphorylation-dephosphorylation kinetics in a crustacean gill (NaMolecular and cellular biochemistry · 2017Article
- Specificity of the connexin W3/4 locus for functional gap junction formation.Channels (Austin, Tex.) · 2016Article
- Determinants of Cx43 Channel Gating and Permeation: The Amino Terminus.Biophysical journal · 2016Article
- Degradation of a connexin40 mutant linked to atrial fibrillation is accelerated.Journal of molecular and cellular cardiology · 2014Article
- Atrial fibrillation-associated connexin40 mutants make hemichannels and synergistically form gap junction channels with novel properties.FEBS letters · 2014Article
- Functional formation of heterotypic gap junction channels by connexins-40 and -43.Channels (Austin, Tex.) · 2014Article
- Interfering amino terminal peptides and functional implications for heteromeric gap junction formation.Frontiers in pharmacology · 2013Article
- Structural organization of intercellular channels II. Amino terminal domain of the connexins: sequence, functional roles, and structure.Biochimica et biophysica acta · 2012Review
- Na⁺,K⁺-ATPase activity in the posterior gills of the blue crab, Callinectes ornatus (Decapoda, Brachyura): modulation of ATP hydrolysis by the biogenic amines spermidine and spermine.The Journal of membrane biology · 2011Article
- Inhibition of cation channels in human erythrocytes by spermine.The Journal of membrane biology · 2010Article
- The N terminus of connexin37 contains an alpha-helix that is required for channel function.The Journal of biological chemistry · 2009Article
- Biological and biophysical properties of vascular connexin channels.International review of cell and molecular biology · 2009Review
- Hindered diffusion through an aqueous pore describes invariant dye selectivity of Cx43 junctions.Biophysical journal · 2008Article
- Effect of transjunctional KCl gradients on the spermine inhibition of connexin40 gap junctions.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spermine blocks connexin40 (Cx40) gap junctions, and two cytoplasmic amino-terminal domain glutamate residues are essential for this inhibitory activity. To further examine the molecular basis for block, we mutated a portion of a basic amino acid (HKH) motif on the Cx40 amino-terminal domain. Replacement of the Cx40 H15 + K16 residues with the Q15 + A16 sequence native to spermine-insensitive connexin43 (Cx43) gap junctions increased the equilibrium dissociation constant (K(d)) and reduced the maximum inhibition by spermine. The corresponding electrical distance (delta) approximation was decreased by about 50%. The transjunctional voltage (V(j))-dependent gating of homotypic Cx40 H15Q + K16A mutant gap junctions was also significantly reduced. The minimum normalized steady-state junctional conductance (G(min)) increased from 0.17 to 0.72, with an increase in the half-inactivation voltage from 48 to 60 mV. However, the unitary junctional conductance (gamma(j); 160 pS) was only slightly altered, and the relative cation/anion conductance and permeability ratios were unchanged from wild-type Cx40 gap junction channels. The relative K(+)/Cl(-) permeability (P(K)/P(Cl)) ratio increased from six to ten when [KCl] was reduced to 25% of normal. These data suggest that the HKH motif at positions 15-17 is important to the conformational structure of the putative voltage sensor and spermine receptor of Cx40, without causing significant alteration of the electrostatic surface charge potentials that contribute to the ion selectivity of this gap junction channel.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.