ArticleJournal of structural and functional genomics2005
Towards miniaturization of a structural genomics pipeline using micro-expression and microcoil NMR.
Article in Journal of structural and functional genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- NMR in structural genomics to increase structural coverage of the protein universe: Delivered by Prof. Kurt Wüthrich on 7 July 2013 at the 38th FEBS Congress in St. Petersburg, Russia.The FEBS journal · 2016Article
- Protein production for structural genomics using E. coli expression.Methods in molecular biology (Clifton, N.J.) · 2014Article
- New LIC vectors for production of proteins from genes containing rare codons.Journal of structural and functional genomics · 2013Article
- NMR-profiles of protein solutions.Biopolymers · 2013Article
- The J-UNIO protocol for automated protein structure determination by NMR in solution.Journal of biomolecular NMR · 2012Article
- NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage-related protein family PF13554.Protein science : a publication of the Protein Society · 2011Article
- Preparation of protein samples for NMR structure, function, and small-molecule screening studies.Methods in enzymology · 2011Article
- The high-throughput protein sample production platform of the Northeast Structural Genomics Consortium.Journal of structural biology · 2010Article
- Rapid, robotic, small-scale protein production for NMR screening and structure determination.Protein science : a publication of the Protein Society · 2010Article
- A microscale protein NMR sample screening pipeline.Journal of biomolecular NMR · 2010Article
- Microscale NMR screening of new detergents for membrane protein structural biology.Journal of the American Chemical Society · 2008Article
- Preservation of protein clefts in comparative models.BMC structural biology · 2008Article
- Small-scale, semi-automated purification of eukaryotic proteins for structure determination.Journal of structural and functional genomics · 2007Article
- Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression.Protein expression and purification · 2007Article
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Authors and funding
7 authors.
Funding
Abstract
In structural genomics centers, nuclear magnetic resonance (NMR) screening is in increasing use as a tool to identify folded proteins that are promising targets for three-dimensional structure determination by X-ray crystallography or NMR spectroscopy. The use of 1D 1H NMR spectra or 2D [1H,15N]-correlation spectroscopy (COSY) typically requires milligram quantities of unlabeled or isotope-labeled protein, respectively. Here, we outline ways towards miniaturization of a structural genomics pipeline with NMR screening for folded globular proteins, using a high-density micro-fermentation device and a microcoil NMR probe. The proteins are micro-expressed in unlabeled or isotope-labeled media, purified, and then subjected to 1D 1H NMR and/or 2D [1H,15N]-COSY screening. To demonstrate that the miniaturization is functioning effectively, we processed nine mouse homologue protein targets and compared the results with those from the "macro-scale" Joint Center of Structural Genomics (JCSG) high-throughput pipeline. The results from the two pipelines were comparable, illustrating that the data were not compromised in the miniaturized approach.
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