ArticleNucleic acids research2005
Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 83 citations in OpenAlex.
- The Promise of Synthetic Biology for Redesigning Plant Architecture.International journal of molecular sciences · 2026Review
- The switch-liker's guide to plant synthetic gene circuits.The Plant journal : for cell and molecular biology · 2025Review
- The TOPLESS corepressor regulates developmental switches in the bryophyte Physcomitrium patens that were critical for plant terrestrialisation.The Plant journal : for cell and molecular biology · 2023Article
- CLAVATA modulates auxin homeostasis and transport to regulate stem cell identity and plant shape in a moss.The New phytologist · 2022Article
- Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella.Plant cell reports · 2022Article
- Targeted Gene Knockouts by Protoplast Transformation in the MossFrontiers in genome editing · 2021Review
- Functional redundancy and divergence of β-carbonic anhydrases in Physcomitrella patens.Planta · 2020Article
- ABA-Induced Vegetative Diaspore Formation inFrontiers in plant science · 2019Article
- The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting inFrontiers in plant science · 2019Article
- Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction.Journal of visualized experiments : JoVE · 2018Article
- Microscopy ofPlant methods · 2017Article
- Testing of Auxotrophic Selection Markers for Use in the MossFrontiers in plant science · 2017Article
- Origin and function of stomata in the moss Physcomitrella patens.Nature plants · 2016Article
- An ancestral stomatal patterning module revealed in the non-vascular land plant Physcomitrella patens.Development (Cambridge, England) · 2016Article
- Genetic Analysis of Physcomitrella patens Identifies ABSCISIC ACID NON-RESPONSIVE, a Regulator of ABA Responses Unique to Basal Land Plants and Required for Desiccation Tolerance.The Plant cell · 2016Article
- Giant peroxisomes in a moss (Physcomitrella patens) peroxisomal biogenesis factor 11 mutant.The New phytologist · 2016Article
- Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Article
- RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella.Nucleic acids research · 2014Article
- Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.DNA repair · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the moss Physcomitrella patens, transforming DNA containing homologous sequences integrates predominantly by homologous recombination with its genomic target. A systematic investigation of the parameters that determine gene targeting efficiency shows a direct relationship between homology length and targeting frequency for replacement vectors (a selectable marker flanked by homologous DNA). Overall homology of only 1 kb is sufficient to achieve a 50% yield of targeted transformants. Targeting may occur through homologous recombination in one arm, accompanied by non-homologous end-joining by the other arm of the vector, or by allele replacement following two homologous recombination events. Allele replacement frequency depends on the symmetry of the targeting vector, being proportional to the length of the shorter arm. Allele replacement may involve insertion of multiple copies of the transforming DNA, accompanied by ectopic insertions at non-homologous sites. Single-copy and single insertions at targeted loci (targeted gene replacements, 'TGR') occur with a frequency of 7-20% of all transformants when the minimum requirements for allele replacement are met. Homologous recombination in Physcomitrella is substantially more efficient than in any multicellular eukaryote, recommending it as the outstanding model for the study of homologous recombination in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.