ArticleJournal of virology2005
Endocytosis and a low-pH step are required for productive entry of equine infectious anemia virus.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Review
- Article
- An Additional Perspective on Proton Pump Inhibitors as Risk Factors for COVID-19.Clinical drug investigation · 2021Article
- Article
- Zika virus: mapping and reprogramming the entry.Cell communication and signaling : CCS · 2019Article
- OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Critical role of leucine-valine change in distinct low pH requirements for membrane fusion between two related retrovirus envelopes.The Journal of biological chemistry · 2012Article
- Filovirus entry: a novelty in the viral fusion world.Viruses · 2012Review
- Retrovirus entry by endocytosis and cathepsin proteases.Advances in virology · 2012Article
- Membrane fusion and cell entry of XMRV are pH-independent and modulated by the envelope glycoprotein's cytoplasmic tail.PloS one · 2012Article
- Cell entry of avian reovirus follows a caveolin-1-mediated and dynamin-2-dependent endocytic pathway that requires activation of p38 mitogen-activated protein kinase (MAPK) and Src signaling pathways as well as microtubules and small GTPase Rab5 protein.The Journal of biological chemistry · 2011Article
- Receptor binding and low pH coactivate oncogenic retrovirus envelope-mediated fusion.Journal of virology · 2009Article
- Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris.Virology journal · 2009Article
- An equine infectious anemia virus variant superinfects cells through novel receptor interactions.Journal of virology · 2008Article
- Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.Virology · 2008Article
- Fusogenicity of Jaagsiekte sheep retrovirus envelope protein is dependent on low pH and is enhanced by cytoplasmic tail truncations.Journal of virology · 2008Article
- Jaagsiekte sheep retrovirus utilizes a pH-dependent endocytosis pathway for entry.Journal of virology · 2008Article
- Equine infectious anemia virus entry occurs through clathrin-mediated endocytosis.Journal of virology · 2008Article
- Entry of Rice dwarf virus into cultured cells of its insect vector involves clathrin-mediated endocytosis.Journal of virology · 2007Article
- Inhibition of betanodavirus infection by inhibitors of endosomal acidification.Archives of virology · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Recently, it has become evident that entry of some retroviruses into host cells is dependent upon a vesicle-localized, low-pH step. The entry mechanism of equine infectious anemia virus (EIAV) has yet to be examined. Here, we demonstrate that wild-type strains of EIAV require a low-pH step for productive entry. Lysosomotropic agents that inhibit the acidification of internal vesicles inhibited productive entry of EIAV. The presence of ammonium chloride (30 mM), monensin (30 microM), or bafilomycin A (50 nM) in the medium dramatically decreased the number of EIAV antigen-positive cells. We found that a low-pH step was required for EIAV infection of tissue culture cell lines as well as primary cells, such as endothelial cells and monocyte-derived macrophages. The ammonium chloride treatment did not reduce virion stability, nor did the treatment prevent virion binding to cells. Consistent with a requirement for a low-pH step, virion infectivity was enhanced more than threefold by brief low-pH treatment following binding of viral particles to permissive cells. A superinfecting variant strain of EIAV, vMA-1c, did not require a low-pH step for productive infection of fibroblasts. However, lysosomotropic agents were inhibitory to vMA-1c infection in the other cell types that vMA-1c infected but did not superinfect, indicating that the entry pathway used by vMA-1c for superinfection abrogates the need for the low-pH step.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.