ArticleAmerican journal of physiology. Cell physiology2006
Stretch increases alveolar epithelial permeability to uncharged micromolecules.
Article in American journal of physiology. Cell physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- On-demand delivery of fibulin-1 protects the basement membrane during cyclic stretching in C. elegans.Developmental cell · 2025Article
- Plastic induced urinary tract disease and dysfunction: a scoping review.Journal of exposure science & environmental epidemiology · 2025Article
- Biological boundary conditions regulate the internalization ofFrontiers in cellular and infection microbiology · 2025Article
- A stretchable human lung-on-chip model of alveolar inflammation for evaluating anti-inflammatory drug response.Bioengineering & translational medicine · 2025Article
- Modulation of the hippo-YAP pathway by cyclic stretch in rat type 2 alveolar epithelial cells-a proof-of-concept study.Frontiers in physiology · 2023Article
- Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.The Journal of cell biology · 2022Article
- Simple graphical approach to investigate differences in transepithelial paracellular leak pathway permeability.Physiological reports · 2022Article
- Advanced human-relevant in vitro pulmonary platforms for respiratory therapeutics.Advanced drug delivery reviews · 2021Review
- The Epithelial Cell Leak Pathway.International journal of molecular sciences · 2021Review
- Characteristics of Passive Solute Transport across Primary Rat Alveolar Epithelial Cell Monolayers.Membranes · 2021Article
- Is Progression of Pulmonary Fibrosis due to Ventilation-induced Lung Injury?American journal of respiratory and critical care medicine · 2019Review
- Medium throughput breathing human primary cell alveolus-on-chip model.Scientific reports · 2018Article
- Protein kinase R-like endoplasmatic reticulum kinase is a mediator of stretch in ventilator-induced lung injury.Respiratory research · 2018Article
- Plasma membrane wounding and repair in pulmonary diseases.American journal of physiology. Lung cellular and molecular physiology · 2017Review
- Simvastatin Treatment Modulates Mechanically-Induced Injury and Inflammation in Respiratory Epithelial Cells.Annals of biomedical engineering · 2016Article
- Regional tidal lung strain in mechanically ventilated normal lungs.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- Superoxide mediates tight junction complex dissociation in cyclically stretched lung slices.Journal of biomechanics · 2016Article
- Modeling the Progression of Epithelial Leak Caused by Overdistension.Cellular and molecular bioengineering · 2016Article
- Local influence of cell viability on stretch-induced permeability of alveolar epithelial cell monolayers.Cellular and molecular bioengineering · 2016Article
- Reproducible uniform equibiaxial stretch of precision-cut lung slices.American journal of physiology. Lung cellular and molecular physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We measured stretch-induced changes in transepithelial permeability in vitro to uncharged tracers 1.5-5.5 A in radius to identify a critical stretch threshold associated with failure of the alveolar epithelial transport barrier. Cultured alveolar epithelial cells were subjected to a uniform cyclic (0.25 Hz) biaxial 12, 25, or 37% change in surface area (DeltaSA) for 1 h. Additional cells served as unstretched controls. Only 37% DeltaSA (100% total lung capacity) produced a significant increase in transepithelial tracer permeability, with the largest increases for bigger tracers. Using the permeability data, we modeled the epithelial permeability in each group as a population of small pores punctuated by occasional large pores. After 37% DeltaSA, increases in paracellular transport were correlated with increases in the radii of both pore populations. Inhibition of protein kinase C and tyrosine kinase activity during stretch did not affect the permeability of stretched cells. In contrast, chelating intracellular calcium and/or stabilizing F-actin during 37% DeltaSA stretch reduced but did not eliminate the stretch-induced increase in paracellular permeability. These results provide the first in vitro evidence that large magnitudes of stretch increase paracellular transport of micromolecules across the alveolar epithelium, partially mediated by intracellular signaling pathways. Our monolayer data are supported by whole lung permeability results, which also show an increase in alveolar permeability at high inflation volumes (20 ml/kg) at the same rate for both healthy and septic lungs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.