ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Src-family kinases stabilize the neuromuscular synapse in vivo via protein interactions, phosphorylation, and cytoskeletal linkage of acetylcholine receptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 59 citations in OpenAlex.
- Protein networking: nicotinic acetylcholine receptors and their protein-protein-associations.Molecular and cellular biochemistry · 2024Review
- Maturation of a postsynaptic domain: Role of small Rho GTPases in organising nicotinic acetylcholine receptor aggregates at the vertebrate neuromuscular junction.Journal of anatomy · 2022Review
- β-Dystroglycan Restoration and Pathology Progression in the DystrophicBiomolecules · 2021Article
- The Laminin-Induced Phosphorylation of PKCδ Regulates AQP4 Distribution and Water Permeability in Rat Astrocytes.Cellular and molecular neurobiology · 2021Article
- The MouseFrontiers in cellular neuroscience · 2020Article
- MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses.The Journal of cell biology · 2019Article
- Beta-2 Adrenergic Receptor Agonists Enhance AChR Clustering in C2C12 Myotubes: Implications for Therapy of Myasthenic Disorders.Journal of neuromuscular diseases · 2018Article
- Protein Tyrosine Signaling and its Potential Therapeutic Implications in Carcinogenesis.Current pharmaceutical design · 2017Review
- Dach2-Hdac9 signaling regulates reinnervation of muscle endplates.Development (Cambridge, England) · 2015Article
- Neuromuscular synapse integrity requires linkage of acetylcholine receptors to postsynaptic intermediate filament networks via rapsyn-plectin 1f complexes.Molecular biology of the cell · 2014Article
- Functional characterization of G-protein-coupled receptors: a bioinformatics approach.Neuroscience · 2014Article
- Muscle-specific kinase (MuSK) autoantibodies suppress the MuSK pathway and ACh receptor retention at the mouse neuromuscular junction.The Journal of physiology · 2014Article
- Early gene expression changes in skeletal muscle from SOD1(G93A) amyotrophic lateral sclerosis animal model.Cellular and molecular neurobiology · 2014Article
- Coronin 6 regulates acetylcholine receptor clustering through modulating receptor anchorage to actin cytoskeleton.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.Skeletal muscle · 2012Article
- The formation of complex acetylcholine receptor clusters requires MuSK kinase activity and structural information from the MuSK extracellular domain.Molecular and cellular neurosciences · 2012Article
- Role of extracellular matrix proteins and their receptors in the development of the vertebrate neuromuscular junction.Developmental neurobiology · 2011Review
- Article
- Time lapse in vivo visualization of developmental stabilization of synaptic receptors at neuromuscular junctions.The Journal of biological chemistry · 2010Article
- Statistical analysis of high-resolution light microscope images reveals effects of cytoskeleton-disrupting drugs on the membrane organization of the nicotinic acetylcholine receptor.The Journal of membrane biology · 2010Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postnatal stabilization and maturation of the postsynaptic membrane are important for development and function of the neuromuscular junction (NMJ), but the underlying mechanisms remain poorly characterized. We examined the role of Src-family kinases (SFKs) in vivo. Electroporation of kinase-inactive Src constructs into soleus muscles of adult mice caused NMJ disassembly: acetylcholine receptor (AChR)-rich areas became fragmented; the topology of nerve terminal, AChRs, and synaptic nuclei was disturbed; and occasionally nerves started to sprout. Electroporation of kinase-overactive Src produced similar but milder effects. We studied the mechanism of SFK action using cultured src(-/-);fyn(-/-) myotubes, focusing on clustering of postsynaptic proteins, their interaction with AChRs, and AChR phosphorylation. Rapsyn and the utrophin-glycoprotein complex were recruited normally into AChR-containing clusters by agrin in src(-/-);fyn(-/-) myotubes. But after agrin withdrawal, clusters of these proteins disappeared rapidly in parallel with AChRs, revealing that SFKs are of general importance in postsynaptic stability. At the same time, AChR interaction with rapsyn and dystrobrevin and AChR phosphorylation decreased after agrin withdrawal from mutant myotubes. Unexpectedly, levels of rapsyn protein were increased in src(-/-);fyn(-/-) myotubes, whereas rapsyn-cytoskeleton interactions were unaffected. The overall cytoskeletal link of AChRs was weak but still strengthened by agrin in mutant cells, consistent with the normal formation but decreased stability of AChR clusters. These data show that correctly balanced activity of SFKs is critical in maintaining adult NMJs in vivo. SFKs hold the postsynaptic apparatus together through stabilization of AChR-rapsyn interaction and AChR phosphorylation. In addition, SFKs control rapsyn levels and AChR-cytoskeletal linkage.
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