ArticleEukaryotic cell2005
snRNA and heterochromatin formation are involved in DNA excision during macronuclear development in stichotrichous ciliates.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Macronuclear development in ciliates, with a focus on nuclear architecture.The Journal of eukaryotic microbiology · 2022Review
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- Review
- The Polycomb protein Ezl1 mediates H3K9 and H3K27 methylation to repress transposable elements in Paramecium.Nature communications · 2019Article
- Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell 'Omics.Genome biology and evolution · 2018Article
- 27nt-RNAs guide histone variant deposition via 'RNA-induced DNA replication interference' and thus transmit parental genome partitioning in Stylonychia.Epigenetics & chromatin · 2018Article
- Programmed Genome Rearrangements in the Ciliate Oxytricha.Microbiology spectrum · 2014Review
- Differential expression of histone H3 genes and selective association of the variant H3.7 with a specific sequence class in Stylonychia macronuclear development.Epigenetics & chromatin · 2014Article
- A permissive chromatin structure is adopted prior to site-specific DNA demethylation of developmentally expressed genes involved in macronuclear differentiation.Epigenetics & chromatin · 2013Article
- RNA-directed epigenetic regulation of DNA rearrangements.Essays in biochemistry · 2010Review
- Alternative processing of scrambled genes generates protein diversity in the ciliate Chilodonella uncinata.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2010Article
- The evolutionary history of histone H3 suggests a deep eukaryotic root of chromatin modifying mechanisms.BMC evolutionary biology · 2010Article
- The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.Eukaryotic cell · 2010Article
- Silencing-associated and meiosis-specific small RNA pathways in Paramecium tetraurelia.Nucleic acids research · 2009Article
- Spatial and temporal plasticity of chromatin during programmed DNA-reorganization in Stylonychia macronuclear development.Epigenetics & chromatin · 2008Article
- The pathway to detangle a scrambled gene.PloS one · 2008Article
- The germ line limited M element of Tetrahymena is targeted for elimination from the somatic genome by a homology-dependent mechanism.Nucleic acids research · 2006Article
- DNA rearrangements directed by non-coding RNAs in ciliates.Wiley interdisciplinary reviews. RNAReview
- New perspectives on the diversification of the RNA interference system: insights from comparative genomics and small RNA sequencing.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several models for specific excision of micronucleus-specific DNA sequences during macronuclear development in ciliates exist. While the template-guided recombination model suggests recombination events resulting in specific DNA excision and reordering of macronucleus-destined sequences (MDS) guided by a template, there is evidence that an RNA interference-related mechanism is involved in DNA elimination in holotrichous ciliates. We describe that in the stichotrichous ciliate Stylonychia, snRNAs homologous to micronucleus-specific sequences are synthesized during macronuclear differentiation. Western and in situ analyses demonstrate that histone H3 becomes methylated at K9 de novo during macronuclear differentiation, and chromatin immunoprecipitation revealed that micronucleus-specific sequences are associated with methylated H3. To link both observations, expression of a PIWI homolog, member of the RNA-induced silencing complex, was silenced. In these cells, the methylated micronucleus-specific histone H3 variant "X" is still present in macronuclear anlagen and no K9 methylation of histone H3 is observed. We suggest that snRNA recruits chromatin-modifying enzymes to sequences to be excised. Based on our and earlier observations, we believe that this mechanism is not sufficient for specific excision of sequences and reordering of MDS in the developing macronucleus and propose a model for internal eliminated sequence excision and MDS reordering in stichotrichous ciliates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.