ArticleEukaryotic cell2005
Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 87 citations in OpenAlex.
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- A genome-to-proteome map reveals how natural variants drive proteome diversity and shape fitness.Science (New York, N.Y.) · 2025Article
- PhpCNature communications · 2025Article
- A genome-to-proteome atlas charts natural variants controlling proteome diversity and forecasts their fitness effects.bioRxiv : the preprint server for biology · 2024Article
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- Genome-wide identification and characterization of flowering genes in Citrus sinensis (L.) Osbeck: a comparison among C. Medica L., C. Reticulata Blanco, C. Grandis (L.) Osbeck and C. Clementina.BMC genomic data · 2024Article
- HapX-mediated H2B deub1 and SreA-mediated H2A.Z deposition coordinate in fungal iron resistance.Nucleic acids research · 2023Article
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- Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.Frontiers in plant science · 2023Article
- Ubiquitin-Conjugating Enzymes Ubc1 and Ubc4 Mediate the Turnover of Hap4, a Master Regulator of Mitochondrial Biogenesis inMicroorganisms · 2022Article
- Role of the Heme Activator Protein Complex in the Sexual Development of Cryptococcus neoformans.mSphere · 2022Article
- Evolution of transcription factor binding through sequence variations and turnover of binding sites.Genome research · 2022Article
- Article
- Yap5 Competes With Hap4 for the Regulation of Iron Homeostasis Genes in the Human PathogenFrontiers in cellular and infection microbiology · 2021Article
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- Transcription factor Hap5 induces gsh2 expression to enhance 2-phenylethanol tolerance and production in an industrial yeast Candida glycerinogenes.Applied microbiology and biotechnology · 2020Article
- The fungal CCAAT-binding complex and HapX display highly variable but evolutionary conserved synergetic promoter-specific DNA recognition.Nucleic acids research · 2020Article
- "Labile" heme critically regulates mitochondrial biogenesis through the transcriptional co-activator Hap4p inThe Journal of biological chemistry · 2020Article
- Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum.Scientific reports · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The CCAAT-binding factor (CBF) is an evolutionarily conserved multimeric transcriptional activator in eukaryotes. In Saccharomyces cerevisiae, the CCAAT-binding factor is composed of four subunits, termed Hap2p, Hap3p, Hap4p, and Hap5p. The Hap2p/Hap3p/Hap5p heterotrimer is the DNA-binding component of the complex that binds to the consensus 5'-CCAAT-3' sequence in the promoter of target genes. The Hap4p subunit contains the transcriptional activation domain necessary for stimulating transcription after interacting with Hap2p/Hap3p/Hap5p. In this report, we demonstrate that Hap2p, Hap3p, and Hap5p assemble via a one-step pathway requiring all three subunits simultaneously, as opposed to the mammalian CCAAT-binding factor which has been shown to assemble via a two-step pathway with CBF-A (Hap3p homolog) and CBF-C (Hap5p homolog) forming a stable dimer before CBF-B (Hap2p homolog) can interact. We have also found that the interaction of Hap4p with Hap2p/Hap3p/Hap5p requires DNA binding as a prerequisite. To further understand the protein-protein and protein-DNA interactions of this transcription factor, we identified the minimal domain of Hap4p necessary for interaction with the Hap2p/Hap3p/Hap5p-DNA complex, and we demonstrate that this domain is sufficient to complement the respiratory deficiency of a hap4Delta mutant and activate transcription when fused with the VP16 activation domain. These studies provide a further understanding of the assembly of the yeast CCAAT-binding factor at target promoters and raise a number of questions concerning the protein-protein and protein-DNA interactions of this multisubunit transcription factor.
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