ArticleGenome biology2005
Transcriptome analysis of antigenic variation in Plasmodium falciparum--var silencing is not dependent on antisense RNA.
Article in Genome biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 67 citations in OpenAlex.
- PfGCN5 is essential for Plasmodium falciparum survival and transmission and regulates Pf H2B.Z acetylation and chromatin structure.Nucleic acids research · 2025Article
- The RNA structurome in the asexual blood stages of malaria pathogenRNA biology · 2021Article
- Human erythrocyte band 3 is a host receptor forBlood · 2019Article
- A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.Cell reports · 2018Article
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- Regulation of PfEMP1-VAR2CSA translation by a Plasmodium translation-enhancing factor.Nature microbiology · 2017Article
- Expansion of Lysine-rich Repeats in Plasmodium Proteins Generates Novel Localization Sequences That Target the Periphery of the Host Erythrocyte.The Journal of biological chemistry · 2016Article
- Plasmodium Helical Interspersed Subtelomeric (PHIST) Proteins, at the Center of Host Cell Remodeling.Microbiology and molecular biology reviews : MMBR · 2016Review
- Antisense long noncoding RNAs regulate var gene activation in the malaria parasite Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Large, rapidly evolving gene families are at the forefront of host-parasite interactions in Apicomplexa.Parasitology · 2015Review
- Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria.Nature · 2014Article
- Noncoding RNAs as emerging regulators of Plasmodium falciparum virulence gene expression.Current opinion in microbiology · 2014Review
- Identified members of the Streptomyces lividans AdpA regulon involved in differentiation and secondary metabolism.BMC microbiology · 2014Article
- Parasite epigenetics and immune evasion: lessons from budding yeast.Epigenetics & chromatin · 2013Article
- Comparative Genomics and Systems Biology of Malaria ParasitesCurrent bioinformatics · 2012Article
- New Agilent platform DNA microarrays for transcriptome analysis of Plasmodium falciparum and Plasmodium berghei for the malaria research community.Malaria journal · 2012Article
- Module-based subnetwork alignments reveal novel transcriptional regulators in malaria parasite Plasmodium falciparum.BMC systems biology · 2012Article
- An erythrocyte cytoskeleton-binding motif in exported Plasmodium falciparum proteins.Eukaryotic cell · 2011Article
- A global transcriptional analysis of Plasmodium falciparum malaria reveals a novel family of telomere-associated lncRNAs.Genome biology · 2011Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlasmodium falciparum, the causative agent of the most severe form of malaria, undergoes antigenic variation through successive presentation of a family of antigens on the surface of parasitized erythrocytes. These antigens, known as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) proteins, are subject to a mutually exclusive expression system, and are encoded by the multigene var family. The mechanism whereby inactive var genes are silenced is poorly understood. To investigate transcriptional features of this mechanism, we conducted a microarray analysis of parasites that were selected to express different var genes by adhesion to chondroitin sulfate A (CSA) or CD36.
resultsIn addition to oligonucleotides for all predicted protein-coding genes, oligonucleotide probes specific to each known var gene of the FCR3 background were designed and added to the microarray, as well as tiled sense and antisense probes for a subset of var genes. In parasites selected for adhesion to CSA, one full-length var gene (var2csa) was strongly upregulated, as were sense RNA molecules emanating from the 3' end of a limited subset of other var genes. No global relationship between sense and antisense production of var genes was observed, but notably, some var genes had coincident high levels of both antisense and sense transcript.
conclusionMutually exclusive expression of PfEMP1 proteins results from transcriptional silencing of non-expressed var genes. The distribution of steady-state sense and antisense RNA at var loci are not consistent with a silencing mechanism based on antisense silencing of inactive var genes. Silencing of var loci is also associated with altered regulation of genes distal to var loci.
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