ArticlePLoS genetics2005
Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin.
Article in PLoS genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 110 citations in OpenAlex.
- The free-living ciliate Tetrahymena pyriformis inactivates engulfed influenza A(H1N1)pdm09 virus via two distinct types of endosomes.Scientific reports · 2025Article
- The Role of Membrane-Bound Extracellular Vesicles During Co-Stimulation and Conjugation in the CiliateMicroorganisms · 2025Article
- The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.iScience · 2024Article
- Myosin-I's motor and actin assembly activation activities are modular and separable in budding yeast clathrin-mediated endocytosis.microPublication biology · 2024Article
- Perspectives on Principles of Cellular Behavior from the Biophysics of Protists.Integrative and comparative biology · 2023Review
- Structure and dynamics of the contractile vacuole complex in Tetrahymena thermophila.Journal of cell science · 2023Article
- TheMolecular biology of the cell · 2023Article
- Intracellular bioaccumulation of the rare earth element Gadolinium in ciliate cells resulting in biogenic particle formation and excretion.Scientific reports · 2023Article
- Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis.Molecular biology of the cell · 2023Article
- Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022Review
- Phagocytic and pinocytic uptake of cholesterol in Tetrahymena thermophila impact differently on gene regulation for sterol homeostasis.Scientific reports · 2021Article
- Article
- Diversification of CORVET tethers facilitates transport complexity inJournal of cell science · 2020Article
- The structural biology of the dynamin-related proteins: New insights into a diverse, multitalented family.Traffic (Copenhagen, Denmark) · 2019Review
- Regulation of Clathrin-Mediated Endocytosis.Annual review of biochemistry · 2018Review
- Resistance to 6-Methylpurine is Conferred by Defective Adenine Phosphoribosyltransferase in Tetrahymena.Genes · 2018Article
- Non-peptide guided auto-secretion of recombinant proteins by super-folder green fluorescent protein in Escherichia coli.Scientific reports · 2017Article
- Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division.Nature communications · 2017Article
- An evolutionary balance: conservation vs innovation in ciliate membrane trafficking.Traffic (Copenhagen, Denmark) · 2017Review
- Diversity and Universality of EndosymbioticFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of molecular elements is unclear, because features in these cells have been defined mainly at the morphological level. Among these ciliate features are structures resembling clathrin-coated, endocytic pits associated with plasma membrane invaginations called parasomal sacs. The combination of genome-wide sequencing in Tetrahymena thermophila with tools for gene expression and replacement has allowed us to examine this pathway in detail. Here we demonstrate that parasomal sacs are sites of clathrin-dependent endocytosis and that AP-2 localizes to these sites. Unexpectedly, endocytosis in Tetrahymena also involves a protein in the dynamin family, Drp1p (Dynamin-related protein 1). While phylogenetic analysis of AP subunits indicates a primitive origin for clathrin-mediated endocytosis, similar analysis of dynamin-related proteins suggests, strikingly, that the recruitment of dynamin-family proteins to the endocytic pathway occurred independently during the course of the ciliate and metazoan radiations. Consistent with this, our functional analysis suggests that the precise roles of dynamins in endocytosis, as well as the mechanisms of targeting, differ in metazoans and ciliates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.