ArticleNucleic acids research2005
An analysis of the feasibility of short read sequencing.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed.
- Influence of Sequencing Technology on Pangenome-Level Analysis and Detection of Antimicrobial Resistance Genes in ESKAPE Pathogens.Open forum infectious diseases · 2025Article
- Review
- Genome-Wide Development and Characterization of Microsatellite Markers in the Great Web-Spinning SawflyEcology and evolution · 2024Article
- Advancements in Genomic and Behavioral Neuroscience Analysis for the Study of Normal and Pathological Brain Function.Frontiers in molecular neuroscience · 2022Review
- Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.BMC genomics · 2019Article
- riboSeed: leveraging prokaryotic genomic architecture to assemble across ribosomal regions.Nucleic acids research · 2018Article
- Improving alignment accuracy on homopolymer regions for semiconductor-based sequencing technologies.BMC genomics · 2016Article
- SNP Mining in Functional Genes from Nonmodel Species by Next-Generation Sequencing: A Case of Flowering, Pre-Harvest Sprouting, and Dehydration Resistant Genes in Wheat.BioMed research international · 2016Article
- Is the whole greater than the sum of its parts? De novo assembly strategies for bacterial genomes based on paired-end sequencing.BMC genomics · 2015Article
- Computational methods for detecting copy number variations in cancer genome using next generation sequencing: principles and challenges.Oncotarget · 2013Review
- Article
- Oases: robust de novo RNA-seq assembly across the dynamic range of expression levels.Bioinformatics (Oxford, England) · 2012Article
- Gene fragmentation in bacterial draft genomes: extent, consequences and mitigation.BMC genomics · 2012Article
- A genome-wide identification analysis of small regulatory RNAs in Mycobacterium tuberculosis by RNA-Seq and conservation analysis.PloS one · 2012Article
- Fast computation and applications of genome mappability.PloS one · 2012Article
- A new strategy for better genome assembly from very short reads.BMC bioinformatics · 2011Article
- Origin of multiple periodicities in the Fourier power spectra of the Plasmodium falciparum genome.BMC genomics · 2011Article
- Building a model: developing genomic resources for common milkweed (Asclepias syriaca) with low coverage genome sequencing.BMC genomics · 2011Article
- Sequence-based physical mapping of complex genomes by whole genome profiling.Genome research · 2011Article
- The uniqueome: a mappability resource for short-tag sequencing.Bioinformatics (Oxford, England) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.