ArticleWorld journal of gastroenterology2005
Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Tumor-Derived Interleukin 35 Promotes Fibrosis in the Tumor Microenvironment of Pancreatic Cancer by Activating Pancreatic Stellate Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Immunodynamic axis of fibroblast-driven neutrophil infiltration in acute pancreatitis: NF-κB-HIF-1α-CXCL1.Cellular & molecular biology letters · 2025Article
- Activation and Regulation of Pancreatic Stellate Cells in Chronic Pancreatic Fibrosis: A Potential Therapeutic Approach for Chronic Pancreatitis.Biomedicines · 2024Review
- Article
- Irreversible and sustained upregulation of endothelin axis during oncogene-associated pancreatic inflammation and cancer.Neoplasia (New York, N.Y.) · 2020Article
- Biology of pancreatic stellate cells-more than just pancreatic cancer.Pflugers Archiv : European journal of physiology · 2017Review
- Pancreatic stellate cell: Pandora's box for pancreatic disease biology.World journal of gastroenterology · 2017Review
- Article
- Human Pancreatic Cancer-Associated Stellate Cells Remain Activated after in vivo Chemoradiation.Frontiers in oncology · 2014Article
- PSCs and GLP-1R: occurrence in normal pancreas, acute/chronic pancreatitis and effect of their activation by a GLP-1R agonist.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Endothelin receptor-A is required for the recruitment of antitumor T cells and modulates chemotherapy induction of cancer stem cells.Cancer biology & therapy · 2013Article
- Gene expression profiling and endothelin in acute experimental pancreatitis.World journal of gastroenterology · 2012Article
- Survivin expression induced by endothelin-1 promotes myofibroblast resistance to apoptosis.The international journal of biochemistry & cell biology · 2012Article
- Therapeutic potential of perineural invasion, hypoxia and desmoplasia in pancreatic cancer.Current pharmaceutical design · 2012Review
- O-GlcNAcylation: a novel pathway contributing to the effects of endothelin in the vasculature.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Review
- CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production.The Journal of biological chemistry · 2010Article
- Shenmai injection inhibiting the extracellular signal regulated kinase-induced human airway smooth muscle proliferation in asthma.Chinese journal of integrative medicine · 2010Article
- Molecular determinants of the profibrogenic effects of endothelin-1 in pancreatic stellate cells.World journal of gastroenterology · 2009Article
- Synergistic growth inhibitory effects of the dual endothelin-1 receptor antagonist bosentan on pancreatic stellate and cancer cells.Digestive diseases and sciences · 2009Article
- Signal transduction in pancreatic stellate cells.Journal of gastroenterology · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimActivated pancreatic stellate cells (PSCs) are implicated in the pathogenesis of pancreatic fibrosis and inflammation. Endothelin-1 (ET-1), which acts through G-protein coupled ET(A) and ET(B) receptors, has several biological activities. We here examined the ability of ET-1 to affect the cell functions of PSCs and the underlying molecular mechanisms.
methodsPSCs were isolated from the pancreas of male Wistar rats after perfusion with collagenase, and cells between passages two and five were used. Expression of ET-1 and ET receptors was assessed by reverse transcription-PCR and immunostaining. Phosphorylation of myosin regulatory light chain (MLC), extracellular-signal regulated kinase (ERK), and Akt was examined by Western blotting. Contraction of PSCs was assessed on hydrated collagen lattices. Cell migration was examined using modified Boyden chambers. Cell proliferation was assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine.
resultsCulture-activated PSCs expressed ET(A) and ET(B) receptors, and ET-1. ET-1 induced phosphorylation of MLC and ERK, but not Akt. ET-1 induced contraction and migration, but did not alter proliferation of PSCs. ET-1-induced contraction was inhibited by an ET(A) receptor antagonist BQ-123 and an ET(B) receptor antagonist BQ-788, whereas migration was inhibited by BQ-788 but not by BQ-123. A Rho kinase inhibitor Y-27632 abolished both contraction and migration.
conclusionET-1 induced contraction and migration of PSCs through ET receptors and activation of Rho-Rho kinase. ET(A) and ET(B) receptors play different roles in the regulation of these cellular functions in response to ET-1.
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