ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2006
Discrete pulses of molting hormone, 20-hydroxyecdysone, during late larval development of Drosophila melanogaster: correlations with changes in gene activity.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed, 181 citations in OpenAlex.
- Genetic and Molecular Regulation of Ecdysone Biosynthesis During Insect Metamorphosis.Insects · 2026Review
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- Mammalian-like steroidogenesis in plants gives rise to endocrine-mimetic cardenolides.Science advances · 2026Article
- Leptin Acts as a Peripheral Tropic Signal to Tune Steroidogenesis.bioRxiv : the preprint server for biology · 2026Article
- Food absence is a cue for metamorphosis in the solitary beeProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Launching the molecular genetic investigation of the ecdysone signaling cascade: a tribute to David S. Hogness (1925-2019).Genetics · 2025Article
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- Triggering and modulation of a complex behavior by a single peptidergic command neuron inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Early attainment of 20-hydroxyecdysone threshold shapes mosquito sexual dimorphism in developmental timing.Nature communications · 2025Article
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- Insect hormone PTTH regulates lifespan through temporal and spatial activation of NF-κB signaling during metamorphosis.bioRxiv : the preprint server for biology · 2024Article
- Synchronizing Drosophila larvae with the salivary gland reporter Sgs3-GFP for discovery of phenotypes in the late third instar stage.Developmental biology · 2024Article
- A dynamical model of growth and maturation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Starvation selection reduces and delays larval ecdysone production and signaling.The Journal of experimental biology · 2023Article
- Steroid hormone regulation of innate immunity in Drosophila melanogaster.PLoS genetics · 2023Review
- Poly(ADP-ribosyl)ating enzymes cooperate to coordinate development.Scientific reports · 2022Article
- The wing imaginal disc.Genetics · 2022Article
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- The steroid hormone ecdysone regulates growth rate in response to oxygen availability.Scientific reports · 2022Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Periodic pulses of the insect steroid molting hormone 20-hydroxyecdysone (20E), acting via its nuclear receptor complex (EcR/USP), control gene expression at many stages throughout Drosophila development. However, during the last larval instar of some lepidopteran insects, subtle changes in titers of ecdysteroids have been documented, including the so-called "commitment peak." This small elevation of 20E reprograms the larva for metamorphosis to the pupa. Similar periods of ecdysteroid immunoreactivity have been observed during the last larval instar of Drosophila. However, due to low amplitude and short duration, along with small body size and staging difficulties, their timing and ecdysteroid composition have remained uncertain. Employing a rigorous regimen of Drosophila culture and a salivary gland reporter gene, Sgs3-GFP, we used RP-HPLC and differential ecdysteroid RIA analysis to determine whole body titers of 20E during the last larval instar. Three small peaks of 20E were observed at 8, 20, and 28 hr following ecdysis, prior to the well-characterized large peak around the time of pupariation. The possible regulation of 20E levels by biosynthetic P450 enzymes and the roles of these early peaks in coordinating gene expression and late larval development are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.