ArticleJournal of plant research2005
Activation tagging approach in a model legume, Lotus japonicus.
Article in Journal of plant research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.Plant physiology · 2022Article
- Jasmonate-Sensitivity-Assisted Screening and Characterization of Nicotine Synthetic Mutants from Activation-Tagged Population of Tobacco (Frontiers in plant science · 2017Article
- Characterization of a potato activation-tagged mutant, nikku, and its partial revertant.Planta · 2015Article
- A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus.PloS one · 2015Article
- Characterization and RNA-seq analysis of underperformer, an activation-tagged potato mutant.Plant molecular biology · 2014Article
- The National BioResource Project (NBRP) Lotus and Glycine in Japan.Breeding science · 2012Article
- Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago truncatula and Lotus japonicus in Bulgaria.Current genomics · 2011Article
- Phenome analysis in plant species using loss-of-function and gain-of-function mutants.Plant & cell physiology · 2009Review
- Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository.Planta · 2009Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We constructed T-DNA insertional lines of a model legume, Lotus japonicus, using a multifunctional vector for gene and exon activation tagging. The vector had the CaMV 35S promoter together with two additional enhancer elements, the start codon, and splice donor and acceptor sites facing the left border, in anticipation of the activation of T-DNA flanking genes and forced expression of flanking exons. The improved transformation technique yielded more than 3,500 lines, including 45 dominant mutant candidates with abnormal phenotypes with respect to aerial parts, nodules, and roots. Among the 44 selected lines, one copy of T-DNA was inserted into the genome of 37 lines (84%). The T-DNA flanking regions of seven lines were isolated by thermal asymmetric interlaced (TAIL)-PCR or reverse transcription (RT)-PCR, and the corresponding genomic clones were analyzed. The transcripts of four genes adjacent to T-DNA out of 11 genes tested were increased in the T(1) generation, demonstrating that gene and exon activation effects by the newly developed tagging vector are heritable. The T-DNA insertional population of L. japonicus will provide legume-specific dominant mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.