ArticleJournal of clinical microbiology2005
Direct detection and identification of Mycobacterium tuberculosis and Mycobacterium bovis in bovine samples by a novel nested PCR assay: correlation with conventional techniques.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Global prevalence and risk factors of bovine tuberculosis in cattle: a systematic review and meta-analysis.Frontiers in veterinary science · 2026Pooled it
- Evidence of mycobacteriaemias and mycobacterial co-infections uncovered in cattle at slaughter using a novel phage-based PhMS-qPCR assay for viable Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis.Irish veterinary journal · 2025Article
- Unseen Threats to Meat Safety: Exposing the Hidden Epidemic to Bovine Tuberculosis in Slaughterhouses.Food science of animal resources · 2025Article
- Review
- Preliminary study of molecular identification of Mycobacterium bovis from cow's milk in Lorestan (Iran).Scientific reports · 2024Article
- Pathogen Detection in Early Phases of Experimental Bovine Tuberculosis.Veterinary sciences · 2024Article
- Unveiling insights into bovine tuberculosis: A comprehensive review.Open veterinary journal · 2024Review
- Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection ofMicrobiology spectrum · 2023Article
- Diagnostic Evaluation of the IS1081-Targeted Real-Time PCR for Detection ofPathogens (Basel, Switzerland) · 2023Article
- Divergent proinflammatory immune responses associated with the differential susceptibility of cattle breeds to tuberculosis.Frontiers in immunology · 2023Article
- Review
- Aptamer Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Real-Time PCR Validation forFrontiers in veterinary science · 2021Article
- A molecular strategy to optimize bovine tuberculosis post-mortem diagnosis and the exposure to Mycobacterium tuberculosis variant bovis.Molecular biology reports · 2020Article
- Culture positivity of smear negative pulmonary and extrapulmonary tuberculosis- A study from North Kerala, India.Journal of family medicine and primary care · 2019Article
- Validation of a Real-Time PCR for the Detection ofFrontiers in veterinary science · 2019Article
- Complement Dependent and Independent Interaction Between Bovine Conglutinin andFrontiers in immunology · 2018Article
- Prevalence of zoonotic tuberculosis and associated risk factors in Central Indian populations.Journal of epidemiology and global health · 2017Article
- Diagnosis of mycobacteria in bovine milk: an overview.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Review
- Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis.Journal of microbiology (Seoul, Korea) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium tuberculosis and M. bovis infect animals and humans. Their epidemiology in developed and developing countries differs, owing to differences in the implementation of preventive measures (World Health Organization, 1999). Identification and differentiation of these closely related mycobacterial species would help to determine the source, reservoirs of infection, and disease burden due to diverse mycobacterial pathogens. The utility of the hupB gene (Rv2986c in M. tuberculosis, or Mb3010c in M. bovis) to differentiate M. tuberculosis and M. bovis was evaluated by a PCR-restriction fragment length polymorphism (RFLP) assay with 56 characterized bovine isolates. The degree of concordance between the PCR-RFLP assay and the microbiological characterization was 99.0% (P < 0.001). A nested PCR (N-PCR) assay was developed, replacing the PCR-RFLP assay for direct detection of M. tuberculosis and M. bovis in bovine samples. The N-PCR products of M. tuberculosis and M. bovis corresponded to 116 and 89 bp, respectively. The detection limit of mycobacterial DNA by N-PCR was 50 fg, equivalent to five tubercle bacilli. M. tuberculosis and/or M. bovis was detected in 55.5% (105/189) of the samples by N-PCR, compared to 9.4% (18/189) by culture. The sensitivities of N-PCR and culture were 97.3 and 29.7, respectively, and their specificities were 22.2 and 77.7%, respectively. The percentages of animals or samples identified as infected with M. tuberculosis or M. bovis by N-PCR and culture reflected the clinical categorizations of the cattle (P of <0.05 to <0.01). Mixed infection by N-PCR was detected in 22 animals, whereas by culture mixed infection was detected in 1 animal.
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