ArticleJournal of clinical microbiology2005
Rapid detection of Mycobacterium tuberculosis in respiratory samples by transcription-reverse transcription concerted reaction with an automated system.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Pooled it
- The Prognostic Value of the Neutrophil-to-lymphocyte Ratio in Older Patients with Pulmonary Tuberculosis.Internal medicine (Tokyo, Japan) · 2026Article
- Endobronchial tuberculosis mimicking malignancy: Lessons from a case report.Respiratory medicine case reports · 2025Article
- Disseminated Tuberculosis with Cholecystitis in a Patient after Cord Blood Transplantation.Internal medicine (Tokyo, Japan) · 2020Article
- Rapid detection of the Mycobacterium tuberculosis complex by use of quenching probe PCR (geneCube).Journal of clinical microbiology · 2012Article
- Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?Journal of clinical microbiology · 2012Article
- Development of a transcription-reverse transcription concerted reaction method for specific detection of human enterovirus 71 from clinical specimens.Journal of clinical microbiology · 2012Article
- Comprehensive multicenter evaluation of a new line probe assay kit for identification of Mycobacterium species and detection of drug-resistant Mycobacterium tuberculosis.Journal of clinical microbiology · 2012Article
- Clinical evaluation of TRCRapid M.TB for detection of Mycobacterium tuberculosis complex in respiratory and nonrespiratory specimens.Journal of clinical microbiology · 2010Article
- Multicenter evaluation of a transcription-reverse transcription concerted assay for rapid detection of mycobacterium tuberculosis complex in clinical specimens.Journal of clinical microbiology · 2009Article
- Establishment of a new method, transcription-reverse transcription concerted reaction, for detection of plasma hnRNP B1 mRNA, a biomarker of lung cancer.Journal of cancer research and clinical oncology · 2008Article
- Carcinoembryonic antigen-producing multiple myeloma detected by a transcription-reverse transcription concerted reaction system.International journal of hematology · 2007Article
- Clinical Features and Prognosis of Nontuberculous Mycobacterial Pleuritis.Respiration; international review of thoracic diseasesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to evaluate the performance of the transcription-reverse transcription concerted (TRC) method for the detection of Mycobacterium tuberculosis complex (MTC) 16S rRNA in clinical respiratory samples for the diagnosis of pulmonary tuberculosis. TRC is a novel method that enables the rapid and the completely homogeneous real-time monitoring of isothermal sequence RNA amplification without any postamplification procedure. The detection limit of the TRC method for MTC was one organism per 100 mul of sputum. The specificity of the method was confirmed by the absence of positive signals for sputum containing 10(6) M. avium or M. kansasii organisms per 100 microl. A total of 201 respiratory samples from patients diagnosed with or suspected of having tuberculosis were tested. Of the 72 MTC culture-positive samples, the TRC method was positive for 52 (sensitivity, 72.2%), whereas the Roche COBAS AMPLICOR PCR was positive for 58 (sensitivity, 80.6%). Both the TRC method and the COBAS AMPLICOR PCR showed no positive identification for any of the 129 culture-negative samples. The percent agreement between the two methods was 95% (191 of 201 samples). The high sensitivity and specificity together with shorter detection time (within 1 h) of the TRC method allow it to be proposed as a useful method for the rapid detection of MTC in respiratory samples.
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