ArticleBiophysical journal2006
Cholesterol depletion induces solid-like regions in the plasma membrane.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 104 citations in OpenAlex.
- Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.bioRxiv : the preprint server for biology · 2026Article
- Statin-induced increase in actin polymerization modulates GPCR dynamics and compartmentalization.Biophysical journal · 2023Article
- Heterogeneous nanoscopic lipid diffusion in the live cell membrane and its dependency on cholesterol.Biophysical journal · 2022Article
- Transbilayer Coupling of Lipids in Cells Investigated by Imaging Fluorescence Correlation Spectroscopy.The journal of physical chemistry. B · 2022Article
- Depletion of the cellular cholesterol content reduces the dynamics of desmosomal cadherins and interferes with desmosomal strength.Histochemistry and cell biology · 2019Article
- Motional dynamics of single Patched1 molecules in cilia are controlled by Hedgehog and cholesterol.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mapping the stochastic sequence of individual ligand-receptor binding events to cellular activation: T cells act on the rare events.Science signaling · 2019Article
- Compartmentalized cAMP Signaling Associated With Lipid Raft and Non-raft Membrane Domains in Adult Ventricular Myocytes.Frontiers in pharmacology · 2018Article
- n-Alcohol Length Governs Shift in LBiophysical journal · 2017Article
- Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state.Nature communications · 2017Article
- Shear-mediated platelet activation in the free flow: Perspectives on the emerging spectrum of cell mechanobiological mechanisms mediating cardiovascular implant thrombosis.Journal of biomechanics · 2017Article
- Article
- Roles for lipid heterogeneity in immunoreceptor signaling.Biochimica et biophysica acta · 2016Review
- Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane.Molecular biology of the cell · 2016Article
- Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.The Journal of biological chemistry · 2015Article
- Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.PLoS neglected tropical diseases · 2014Article
- Oxygen depletion speeds and simplifies diffusion in HeLa cells.Biophysical journal · 2014Article
- Coupling between endocytosis and sphingosine kinase 1 recruitment.Nature cell biology · 2014Article
- Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes.Traffic (Copenhagen, Denmark) · 2014Article
- Transient cholesterol effects on nicotinic acetylcholine receptor cell-surface mobility.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glycosylphosphatidylinositol-linked and transmembrane major histocompatibility complex (MHC) class II I-E(k) proteins, as well as N-(6-tetramethylrhodaminethiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Tritc-DHPE), are used as probes to determine the effect of cholesterol concentration on the organization of the plasma membrane at temperatures in the range 22 degrees C-42 degrees C. Cholesterol depletion caused a decrease in the diffusion coefficients for the MHC II proteins and also for a slow fraction of the Tritc-DHPE population. At 37 degrees C, reduction of the total cell cholesterol concentration results in a smaller suppression of the translational diffusion for I-E(k) proteins (twofold) than was observed in earlier work at 22 degrees C (five sevenfold) Vrljic, M., S. Y. Nishimura, W. E. Moerner, and H. M. McConnell. 2005. Biophys. J. 88:334-347. At 37 degrees C, the diffusion of both I-E(k) proteins is Brownian (0.9 < alpha-parameter < 1.1). More than 99% of the protein population diffuses homogeneously when imaged at 65 frames per s. As the temperature is raised from 22 degrees C to 42 degrees C, a change in activation energy is seen at approximately 35 degrees C in the Arrhenius plots. Cytoskeletal effects appear to be minimal. These results are consistent with a previously described model of solid-like domain formation in the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.