ArticleJournal of the American Society for Mass Spectrometry2005
Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins.
Article in Journal of the American Society for Mass Spectrometry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 58 citations in OpenAlex.
- Analysis of RNA and Its Modifications.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- Advances in mRNA 5-methylcytosine modifications: Detection, effectors, biological functions, and clinical relevance.Molecular therapy. Nucleic acids · 2021Review
- Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry.European biophysics journal : EBJ · 2019Review
- Dissecting human skeletal muscle troponin proteoforms by top-down mass spectrometry.Journal of muscle research and cell motility · 2015Article
- Profiling of integral membrane proteins and their post translational modifications using high-resolution mass spectrometry.Methods (San Diego, Calif.) · 2011Review
- Top-down quantitative proteomics identified phosphorylation of cardiac troponin I as a candidate biomarker for chronic heart failure.Journal of proteome research · 2011Article
- Phosphorylation, but not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac troponin T.Biochemistry · 2011Article
- A preferred AMPK phosphorylation site adjacent to the inhibitory loop of cardiac and skeletal troponin I.Protein science : a publication of the Protein Society · 2011Article
- Unusual fragmentation of β-linked peptides by ExD tandem mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
- Deciphering modifications in swine cardiac troponin I by top-down high-resolution tandem mass spectrometry.Journal of the American Society for Mass Spectrometry · 2010Article
- Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociation.Molecular & cellular proteomics : MCP · 2010Article
- Glutamine deamidation: differentiation of glutamic acid and gamma-glutamic acid in peptides by electron capture dissociation.Analytical chemistry · 2010Article
- Charge remote fragmentation in electron capture and electron transfer dissociation.Journal of the American Society for Mass Spectrometry · 2010Article
- Identification of aspartic and isoaspartic acid residues in amyloid beta peptides, including Abeta1-42, using electron-ion reactions.Analytical chemistry · 2009Article
- Top-down high-resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Acyl peptide hydrolase degrades monomeric and oligomeric amyloid-beta peptide.Molecular neurodegeneration · 2009Article
- CD1c bypasses lysosomes to present a lipopeptide antigen with 12 amino acids.The Journal of experimental medicine · 2009Article
- The spontaneous loss of coherence catastrophe in Fourier transform ion cyclotron resonance mass spectrometry.Journal of the American Society for Mass Spectrometry · 2009Article
- The effect of fixed charge modifications on electron capture dissociation.Journal of the American Society for Mass Spectrometry · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The use of a new electrospray qQq Fourier transform ion cyclotron mass spectrometer (qQq-FTICR MS) instrument for biologic applications is described. This qQq-FTICR mass spectrometer was designed for the study of post-translationally modified proteins and for top-down analysis of biologically relevant protein samples. The utility of the instrument for the analysis of phosphorylation, a common and important post-translational modification, was investigated. Phosphorylation was chosen as an example because it is ubiquitous and challenging to analyze. In addition, the use of the instrument for top-down sequencing of proteins was explored since this instrument offers particular advantages to this approach. Top-down sequencing was performed on different proteins, including commercially available proteins and biologically derived samples such as the human E2 ubiquitin conjugating enzyme, UbCH10. A good sequence tag was obtained for the human UbCH10, allowing the unambiguous identification of the protein. The instrument was built with a commercially produced front end: a focusing rf-only quadrupole (Q0), followed by a resolving quadrupole (Q1), and a LINAC quadrupole collision cell (Q2), in combination with an FTICR mass analyzer. It has utility in the analysis of samples found in substoichiometric concentrations, as ions can be isolated in the mass resolving Q1 and accumulated in Q2 before analysis in the ICR cell. The speed and efficacy of the Q2 cooling and fragmentation was demonstrated on an LCMS-compatible time scale, and detection limits for phosphopeptides in the 10 amol/muL range (pM) were demonstrated. The instrument was designed to make several fragmentation methods available, including nozzle-skimmer fragmentation, Q2 collisionally activated dissociation (Q2 CAD), multipole storage assisted dissociation (MSAD), electron capture dissociation (ECD), infrared multiphoton induced dissociation (IRMPD), and sustained off resonance irradiation (SORI) CAD, thus allowing a variety of MS(n) experiments. A particularly useful aspect of the system was the use of Q1 to isolate ions from complex mixtures with narrow windows of isolation less than 1 m/z. These features enable top-down protein analysis experiments as well structural characterization of minor components of complex mixtures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.