ArticlePlant molecular biology2005
Dual DNA binding property of ABA insensitive 3 like factors targeted to promoters responsive to ABA and auxin.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 66 citations in OpenAlex.
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- Protein Levels of Several Arabidopsis Auxin Response Factors Are Regulated by Multiple Factors and ABA PromotesInternational journal of molecular sciences · 2020Article
- Identification, Gene Structure, and Expression ofFrontiers in plant science · 2020Article
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- The sHSP22 Heat Shock Protein Requires the ABI1 Protein Phosphatase to Modulate Polar Auxin Transport and Downstream Responses.Plant physiology · 2018Article
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- Evolutionary Analysis of theFrontiers in plant science · 2017Article
- Ectopic expression of UGT75D1, a glycosyltransferase preferring indole-3-butyric acid, modulates cotyledon development and stress tolerance in seed germination of Arabidopsis thaliana.Plant molecular biology · 2016Article
- SFGD: a comprehensive platform for mining functional information from soybean transcriptome data and its use in identifying acyl-lipid metabolism pathways.BMC genomics · 2014Article
- Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).PloS one · 2013Article
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- Template-based structure prediction and classification of transcription factors in Arabidopsis thaliana.Protein science : a publication of the Protein Society · 2012Article
- Identification of rice transcription factors associated with drought tolerance using the Ecotilling method.PloS one · 2012Article
- Over-represented promoter motifs in abiotic stress-induced DREB genes of rice and sorghum and their probable role in regulation of gene expression.Plant signaling & behavior · 2010Article
- The conserved splicing factor SUA controls alternative splicing of the developmental regulator ABI3 in Arabidopsis.The Plant cell · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ABA responsive ABI3 and the auxin responsive ARF family of transcription factors bind the CATGCATG (Sph) and TGTCTC core motifs in ABA and auxin response elements (ABRE and AuxRE), respectively. Several evidences indicate ABI3s to act downstream to auxin too. Because DNA binding domain of ABI3s shows significant overlap with ARFs we enquired whether auxin responsiveness through ABI3s could be mediated by their binding to canonical AuxREs. Investigations were undertaken through in vitro gel mobility shift assays (GMSA) using the DNA binding domain B3 of PvAlf (Phaseolus vulgaris ABI3 like factor) and upstream regions of auxin responsive gene GH3 (-267 to -141) and ABA responsive gene Em (-316 to -146) harboring AuxRE and ABRE, respectively. We demonstrate that B3 domain of PvAlf could bind AuxRE only when B3 was associated with its flanking domain B2 (B2B3). Such strict requirement of B2 domain was not observed with ABRE, where B3 could bind with or without being associated with B2. This dual specificity in DNA binding of ABI3s was also demonstrated with nuclear extracts of cultured cells of Arachis hypogea. Supershift analysis of ABRE and AuxRE bound nuclear proteins with antibodies raised against B2B3 domains of PvAlf revealed that ABI3 associated complexes were detectable in association with both cis elements. Competition GMSA confirmed the same complexes to bind ABRE and AuxRE. This dual specificity of ABI3 like factors in DNA binding targeted to natural promoters responsive to ABA and auxin suggests them to have a potential role in conferring crosstalk between these two phytohormones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.