ArticlePlant molecular biology2005
Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 120 citations in OpenAlex.
- Meta-analysis of heat- and chemically upregulated chaperone genes in plant and human cells.Cell stress & chaperones · 2011Pooled it
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Infrared laser-induced gene expression in single cells characterized by quantitative imaging in Physcomitrium patens.Communications biology · 2024Article
- An Engineered Heat-Inducible Expression System for the Production of Casbene inInternational journal of molecular sciences · 2023Article
- Design of an Arabidopsis thaliana reporter line to detect heat-sensing and signaling mutants.Plant methods · 2023Article
- DELLA proteins regulate spore germination and reproductive development in Physcomitrium patens.The New phytologist · 2023Article
- Article
- Process Engineering of Biopharmaceutical Production in Moss BioreactorsFrontiers in bioengineering and biotechnology · 2022Article
- Quantitative proteomic analysis to capture the role of heat-accumulated proteins in moss plant acquired thermotolerance.Plant, cell & environment · 2021Article
- Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens.International journal of molecular sciences · 2020Article
- Efficient and modular CRISPR-Cas9 vector system forPlant direct · 2019Article
- Short- and Long-Term Effects of UVA on Arabidopsis Are Mediated by a Novel cGMP Phosphodiesterase.Current biology : CB · 2019Article
- Physcomitrella patens, a versatile synthetic biology chassis.Plant cell reports · 2018Review
- An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress.Frontiers in plant science · 2018Review
- Combination of the EndogenousFrontiers in plant science · 2017Article
- An ancient and conserved function for Armadillo-related proteins in the control of spore and seed germination by abscisic acid.The New phytologist · 2016Article
- Characterization of 5'UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation.Cell stress & chaperones · 2016Article
- The isoquinoline alkaloid sanguinarine which inhibits chaperone activity enhances the production of heat shock proteins inPlant biotechnology (Tokyo, Japan) · 2016Article
- The Role of the Membrane-Initiated Heat Shock Response in Cancer.Frontiers in molecular biosciences · 2016Review
- Moss-made pharmaceuticals: from bench to bedside.Plant biotechnology journal · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to express tightly controlled amounts of endogenous and recombinant proteins in plant cells is an essential tool for research and biotechnology. Here, the inducibility of the soybean heat-shock Gmhsp17.3B promoter was addressed in the moss Physcomitrella patens, using beta-glucuronidase (GUS) and an F-actin marker (GFP-talin) as reporter proteins. In stably transformed moss lines, Gmhsp17.3B-driven GUS expression was extremely low at 25 degrees C. In contrast, a short non-damaging heat-treatment at 38 degrees C rapidly induced reporter expression over three orders of magnitude, enabling GUS accumulation and the labelling of F-actin cytoskeleton in all cell types and tissues. Induction levels were tightly proportional to the temperature and duration of the heat treatment, allowing fine-tuning of protein expression. Repeated heating/cooling cycles led to the massive GUS accumulation, up to 2.3% of the total soluble proteins. The anti-inflammatory drug acetyl salicylic acid (ASA) and the membrane-fluidiser benzyl alcohol (BA) also induced GUS expression at 25 degrees C, allowing the production of recombinant proteins without heat-treatment. The Gmhsp17.3B promoter thus provides a reliable versatile conditional promoter for the controlled expression of recombinant proteins in the moss P. patens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.