ArticleNucleic acids research2005
Tracking of microinjected DNA in live cells reveals the intracellular behavior and elimination of extrachromosomal genetic material.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Single-molecule FRET and tracking of transfected biomolecules in living cells.Biophysical journal · 2026Article
- Vector-free DNA transfection by nuclear envelope mechanoporation.Lab on a chip · 2026Article
- The intracellular visualization of exogenous DNA in fluorescence microscopy.Drug delivery and translational research · 2024Review
- Gene duplication and deletion caused by over-replication at a fork barrier.Nature communications · 2023Article
- Revisiting characteristics of oncogenic extrachromosomal DNA as mobile enhancers on neuroblastoma and glioma cancers.Cancer cell international · 2022Review
- Preventing excess replication origin activation to ensure genome stability.Trends in genetics : TIG · 2022Review
- Review
- Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo.Advanced biosystems · 2020Article
- Extrachromosomal Circular DNA: Current Knowledge and Implications for CNS Aging and Neurodegeneration.International journal of molecular sciences · 2020Review
- Generation and maintenance of acentric stable double minutes from chromosome arms in inter-species hybrid cells.BMC molecular and cell biology · 2019Article
- Article
- An evolving function of DNA-containing exosomes in chemotherapy-induced immune response.Cell research · 2017Article
- Asymmetric partitioning of transfected DNA during mammalian cell division.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- RPA and Rad51 constitute a cell intrinsic mechanism to protect the cytosol from self DNA.Nature communications · 2016Article
- Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy.Scientific reports · 2015Article
- The precarious prokaryotic chromosome.Journal of bacteriology · 2014Review
- Polyplex-induced cytosolic nuclease activation leads to differential transgene expression.Molecular pharmaceutics · 2013Article
- Cyclic stretch of alveolar epithelial cells alters cytoskeletal micromechanics.Biotechnology and bioengineering · 2011Article
- Generation of micronuclei during interphase by coupling between cytoplasmic membrane blebbing and nuclear budding.PloS one · 2011Article
- Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings.International journal of molecular sciences · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We here addressed the basic question, how does extrachromosomal DNA behave when it is placed in the nuclear or the cytoplasmic environment and how is it eliminated? To do this, we tracked microinjected DNA molecules in live cells. In the cytoplasm, the diffusion of microinjected DNA was inhibited in a size- and linearity-dependent manner, probably by the intermediate filament. This was followed by the rapid disappearance of the DNA fluorescent signal. In the nucleus, the diffusion was also dependent on the size of the molecule and was accompanied by the aggregation of the DNA. The aggregation may be due to a putative DNA-binding molecule, whose level is high during the G1 phase. Surprisingly, the injected DNA could move across the nuclear membrane and appeared in the cytoplasm, which suggests the presence of a transport system. The intracytoplasmic behavior and the elimination of such DNA was obviously different from the DNA that was directly injected at the cytoplasm. The DNA remaining in the nucleus appeared to be stable and persisted in the nucleus or, after cell division, in the cytoplasm, for more than one cell cycle. These findings provide a novel and basic understanding of the behavior and elimination of a wide variety of extrachromosomal genetic material.
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