ArticleNucleic acids research2005
In vitro non-viral gene delivery with nanofibrous scaffolds.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Collagen-Coated Hyperelastic Bone Promotes Osteoblast Adhesion and Proliferation.Materials (Basel, Switzerland) · 2023Article
- Electrospun-Fibrous-Architecture-Mediated Non-Viral Gene Therapy Drug Delivery in Regenerative Medicine.Polymers · 2022Review
- The biological applications of DNA nanomaterials: current challenges and future directions.Signal transduction and targeted therapy · 2021Review
- Electrospun Nanofibers for Cancer Therapy.Advances in experimental medicine and biology · 2021Review
- Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications.Frontiers in bioengineering and biotechnology · 2020Review
- The Therapeutic Potential of MicroRNAs as Orthobiologics for Skeletal Fractures.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Review
- Electrospinning Nanofibers for Therapeutics Delivery.Nanomaterials (Basel, Switzerland) · 2019Review
- Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells.Stem cells international · 2016Article
- Fabricating Superhydrophobic Polymeric Materials for Biomedical Applications.Journal of visualized experiments : JoVE · 2015Article
- Electrospinning strategies of drug-incorporated nanofibrous mats for wound recovery.Drug delivery and translational research · 2015Review
- Electrospun nanofibers as versatile interfaces for efficient gene delivery.Journal of biological engineering · 2014Review
- Physical non-viral gene delivery methods for tissue engineering.Annals of biomedical engineering · 2013Review
- Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering.International journal of nanomedicine · 2012Article
- Cdk2 silencing via a DNA/PCL electrospun scaffold suppresses proliferation and increases death of breast cancer cells.PloS one · 2012Article
- A short review: Recent advances in electrospinning for bone tissue regeneration.Journal of tissue engineering · 2012Article
- Thin-layer hydroxyapatite deposition on a nanofiber surface stimulates mesenchymal stem cell proliferation and their differentiation into osteoblasts.Journal of biomedicine & biotechnology · 2012Article
- Effective gene delivery to mesenchymal stem cells based on the reverse transfection and three-dimensional cell culture system.Pharmaceutical research · 2011Article
- Bioactive electrospun scaffolds delivering growth factors and genes for tissue engineering applications.Pharmaceutical research · 2011Review
- Nano-fibrous tissue engineering scaffolds capable of growth factor delivery.Pharmaceutical research · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular and intracellular barriers typically prevent non-viral gene vectors from having an effective transfection efficiency. Formulation of a gene delivery vehicle that can overcome the barriers is a key step for successful tissue regeneration. We have developed a novel core-shelled DNA nanoparticle by invoking solvent-induced condensation of plasmid DNA (beta-galactosidase or GFP) in a solvent mixture [94% N,N-dimethylformamide (DMF) + 6% 1x TE buffer] and subsequent encapsulation of the condensed DNA globule in a triblock copolymer, polylactide-poly(ethylene glycol)-polylactide (L8E78L8), in the same solvent environment. The polylactide shell protects the encapsulated DNA from degradation during electrospinning of a mixture of encapsulated DNA nanoparticles and biodegradable PLGA (a random copolymer of lactide and glycolide) to form a nanofibrous non-woven scaffold using the same solution mixture. The bioactive plasmid DNA can then be released in an intact form from the scaffold with a controlled release rate and transfect cells in vitro.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.