ArticleApplied and environmental microbiology2005
Proteomic signature of Lactococcus lactis NCDO763 cultivated in milk.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 79 citations in OpenAlex.
- Milk Lipids as Bioactive Modulators of the Bacterial Proteome: Mechanisms Linking Dairy Management to Microbial Performance.Animals : an open access journal from MDPI · 2026Review
- A bacteriolysin of Lactococcus carnosus is potentially involved in mediating contact-dependent antagonism against Listeria monocytogenes.Scientific reports · 2025Article
- Comprehensive characterization of γ-aminobutyric acid (GABA) production byFrontiers in microbiology · 2024Article
- Proteomic perspectives on thermotolerant microbes: an updated review.Molecular biology reports · 2022Review
- Article
- Effects of Protein, Calcium, and pH on Gene Transcription, Cell-Envelope Peptidase Activity ofFoods (Basel, Switzerland) · 2021Article
- Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria.Molecules (Basel, Switzerland) · 2021Review
- Bacterial Diversity Analysis and Evaluation Proteins Hydrolysis During the Acid Whey and Fish Waste Fermentation.Microorganisms · 2021Article
- Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label-free quantitative proteomics.Microbial biotechnology · 2019Article
- YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.Scientific reports · 2017Article
- Integrating highly quantitative proteomics and genome-scale metabolic modeling to study pH adaptation in the human pathogenNPJ systems biology and applications · 2016Article
- Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus.Journal of Zhejiang University. Science. B · 2015Article
- Using a genome-scale metabolic model of Enterococcus faecalis V583 to assess amino acid uptake and its impact on central metabolism.Applied and environmental microbiology · 2015Article
- Evolution of microbiological analytical methods for dairy industry needs.Frontiers in microbiology · 2014Review
- Growth phase-dependent proteomes of the Malaysian isolated Lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis.TheScientificWorldJournal · 2014Article
- Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution.Genome research · 2012Article
- Article
- Dynamic analysis of the Lactococcus lactis transcriptome in cheeses made from milk concentrated by ultrafiltration reveals multiple strategies of adaptation to stresses.Applied and environmental microbiology · 2011Article
- The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9.Journal of bacteriology · 2009Article
- Gene expression profiling of Listeria monocytogenes strain F2365 during growth in ultrahigh-temperature-processed skim milk.Applied and environmental microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have compared the proteomic profiles of L. lactis subsp. cremoris NCDO763 growing in the synthetic medium M17Lac, skim milk microfiltrate (SMM), and skim milk. SMM was used as a simple model medium to reproduce the initial phase of growth of L. lactis in milk. To widen the analysis of the cytoplasmic proteome, we used two different gel systems (pH ranges of 4 to 7 and 4.5 to 5.5), and the proteins associated with the cell envelopes were also studied by two-dimensional electrophoresis. In the course of the study, we analyzed about 800 spots and identified 330 proteins by mass spectrometry. We observed that the levels of more than 50 and 30 proteins were significantly increased upon growth in SMM and milk, respectively. The large redeployment of protein synthesis was essentially associated with an activation of pathways involved in the metabolism of nitrogenous compounds: peptidolytic and peptide transport systems, amino acid biosynthesis and interconversion, and de novo biosynthesis of purines. We also showed that enzymes involved in reactions feeding the purine biosynthetic pathway in one-carbon units and amino acids have an increased level in SMM and milk. The analysis of the proteomic data suggested that the glutamine synthetase (GS) would play a pivotal role in the adaptation to SMM and milk. The analysis of glnA expression during growth in milk and the construction of a glnA-defective mutant confirmed that GS is an essential enzyme for the development of L. lactis in dairy media. This analysis thus provides a proteomic signature of L. lactis, a model lactic acid bacterium, growing in its technological environment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.