ArticleApplied and environmental microbiology2005
Development and application of real-time PCR assays for quantification of genes encoding tetracycline resistance.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed.
- Improving rumen ecology and microbial population by dried rumen digesta in beef cattle.Tropical animal health and production · 2015Trial
- Evaluation of qPCR for QuantifyingAntibiotics (Basel, Switzerland) · 2026Article
- Optimizing goat growth and rumen function with monosodium glutamate byproduct-treated rice straw in total mixed rations.Veterinary world · 2025Article
- Detecting antibiotic resistance: classical, molecular, advanced bioengineering, and AI-enhanced approaches.Frontiers in microbiology · 2025Review
- Connecting the Dots: Livestock Animals as Missing Links in the Chain of Microplastic Contamination and Human Health.Animals : an open access journal from MDPI · 2024Review
- Interactions betweenJournal of animal science and technology · 2023Article
- Biosolids for safe land application: does wastewater treatment plant size matters when considering antibiotics, pollutants, microbiome, mobile genetic elements and associated resistance genes?Environmental microbiology · 2022Article
- Sodium nitrate has no detrimental effect on milk fatty acid profile and rumen bacterial population in water buffaloes.AMB Express · 2022Article
- Antibiotic resistance, antimicrobial residues, and bacterial community diversity in pasture-raised poultry, swine, and beef cattle manures.Journal of animal science · 2021Article
- Association of ionophores, yeast, and bacterial probiotics alters the abundance of ruminal microbial species of pasture intensively finished beef cattle.Tropical animal health and production · 2021Article
- Stochasticity in microbiology: managing unpredictability to reach the Sustainable Development Goals.Microbial biotechnology · 2020Article
- Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs.BMC microbiology · 2019Article
- Dynamics of oxytetracycline and resistance genes in soil under long-term intensive compost fertilization in Northern China.Environmental science and pollution research international · 2019Article
- Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics.AMB Express · 2019Article
- Ruminal Microbial Populations and Fermentation Characteristics in Beef Cattle Grazing Tropical Forage in Dry Season and Supplemented with Different Protein Levels.Current microbiology · 2019Article
- Effects of oregano essential oil on the ruminal pH and microbial population of sheep.PloS one · 2019Article
- Quantitative qPCR Analysis of Ruminal Microorganisms in Beef Cattle Grazing in Pastures in the Rainy Season and Supplemented with Different Protein Levels.Current microbiology · 2018Article
- Occurrence and Distribution of Tetracycline Antibiotics and Resistance Genes in Longshore Sediments of the Three Gorges Reservoir, China.Frontiers in microbiology · 2018Article
- Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts?Frontiers in microbiology · 2018Article
- Comparison of antimicrobial resistance genes in feedlots and urban wastewater.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here the development, validation, and use of three real-time PCR assays to quantify the abundance of the following three groups of tetracycline resistance genes: tet(A) and tet(C); tet(G); and tet genes encoding ribosomal protection proteins, including tet(M), tet(O), tetB(P), tet(Q), tet(S), tet(T), and tet(W). The assays were validated using known numbers of sample-derived tet gene templates added to microbiome DNA. These assays are both precise and accurate over at least 6 log tet gene copies. New tet gene variants were also identified from cloned tet amplicons as part of this study. The utility of these real-time PCR assays was demonstrated by quantifying the three tet gene groups present in bovine and swine manures, composts of swine manure, lagoons of hog house effluent, and samples from an Ekokan upflow biofilter system treating hog house effluent. The bovine manures were found to contain fewer copies of all three groups of tet genes than the swine manures. The composts of swine manures had substantially reduced tet gene abundance (up to 6 log), while lagoon storage or the upflow biofilter had little effect on tet gene abundance. These results suggest that the method of manure storage and treatment may have a substantial impact on the persistence and dissemination of tet genes in agricultural environments. These real-time PCR assays provide rapid, quantitative, cultivation-independent measurements of 10 major classes of tet genes, which should be useful for ecological studies of antibiotic resistance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.