ArticleApplied and environmental microbiology2005
Cloning, biochemical properties, and distribution of mycobacterial haloalkane dehalogenases.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 53 citations in OpenAlex.
- Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA.International journal of molecular sciences · 2021Article
- A Novel Mini Protein Design of Haloalkane Dehalogenase.Molecular biotechnology · 2019Article
- Ylehd, an epoxide hydrolase with promiscuous haloalkane dehalogenase activity from tropical marine yeast Yarrowia lipolytica is induced upon xenobiotic stress.Scientific reports · 2017Article
- Discovery of Novel Haloalkane Dehalogenase Inhibitors.Applied and environmental microbiology · 2016Article
- Biochemical characterization of a haloalkane dehalogenase DadB from Alcanivorax dieselolei B-5.PloS one · 2014Article
- Biochemical characterization of a novel haloalkane dehalogenase from a cold-adapted bacterium.Applied and environmental microbiology · 2012Article
- Isomeric mono-, di-, and tri-bromobenzo-1H-triazoles as inhibitors of human protein kinase CK2α.PloS one · 2012Article
- Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58.Applied and environmental microbiology · 2011Article
- Sequence- and activity-based screening of microbial genomes for novel dehalogenases.Microbial biotechnology · 2010Article
- Biochemical characterization of haloalkane dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily.Applied and environmental microbiology · 2009Article
- Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Biodegradation of bis(2-chloroethyl) ether by Xanthobacter sp. strain ENV481.Applied and environmental microbiology · 2007Article
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haloalkane dehalogenases are enzymes that catalyze the cleavage of the carbon-halogen bond by a hydrolytic mechanism. Genomes of Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterized haloalkane dehalogenases. We describe here the cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis 5033/66 and demonstrate the dehalogenase activity of their translation products. Both of these genes are widely distributed among species of the M. tuberculosis complex, including M. bovis, M. bovis BCG, M. africanum, M. caprae, M. microti, and M. pinnipedii, as shown by the PCR screening of 48 isolates from various hosts. DmbA and DmbB proteins were heterologously expressed in Escherichia coli and purified to homogeneity. The DmbB protein had to be expressed in a fusion with thioredoxin to obtain a soluble protein sample. The temperature optimum of DmbA and DmbB proteins determined with 1,2-dibromoethane is 45 degrees C. The melting temperature assessed by circular dichroism spectroscopy of DmbA is 47 degrees C and DmbB is 57 degrees C. The pH optimum of DmbA depends on composition of a buffer with maximal activity at 9.0. DmbB had a single pH optimum at pH 6.5. Mycobacteria are currently the only genus known to carry more than one haloalkane dehalogenase gene, although putative haloalkane dehalogenases can be inferred in more then 20 different bacterial species by comparative genomics. The evolution and distribution of haloalkane dehalogenases among mycobacteria is discussed.
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