ArticleApplied and environmental microbiology2005
Prediction of the adaptability of Pseudomonas putida DOT-T1E to a second phase of a solvent for economically sound two-phase biotransformations.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Hydrochar from Agricultural Waste as a Biobased Support Matrix Enhances the Bacterial Degradation of Diethyl Phthalate.Molecules (Basel, Switzerland) · 2025Article
- Cross-feeding drives degradation of phthalate ester plasticizers in a bacterial consortium.Frontiers in microbiology · 2025Article
- Assessment of New and Genome-ReducedMicroorganisms · 2023Article
- Mutagenesis and Adaptation of the Psychrotrophic FungusMolecules (Basel, Switzerland) · 2020Article
- Effects of limonene, n-decane and n-decanol on growth and membrane fatty acid composition of the microalga Botryococcus braunii.AMB Express · 2018Article
- Immediate response mechanisms of Gram-negative solvent-tolerant bacteria to cope with environmental stress: cis-trans isomerization of unsaturated fatty acids and outer membrane vesicle secretion.Applied microbiology and biotechnology · 2018Review
- Growth of Pseudomonas taiwanensis VLB120∆C biofilms in the presence of n-butanol.Microbial biotechnology · 2017Article
- Article
- Making variability less variable: matching expression system and host for oxygenase-based biotransformations.Journal of industrial microbiology & biotechnology · 2015Article
- Isolation of butanol- and isobutanol-tolerant bacteria and physiological characterization of their butanol tolerance.Applied and environmental microbiology · 2013Article
- Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome.Microbial biotechnology · 2013Article
- Adaptation of the hydrocarbonoclastic bacterium Alcanivorax borkumensis SK2 to alkanes and toxic organic compounds: a physiological and transcriptomic approach.Applied and environmental microbiology · 2013Article
- Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.Applied and environmental microbiology · 2012Article
- The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.Microbial biotechnology · 2012Article
- Selected Pseudomonas putida strains able to grow in the presence of high butanol concentrations.Applied and environmental microbiology · 2009Article
- Biodegradation of 2-ethylhexyl nitrate by Mycobacterium austroafricanum IFP 2173.Applied and environmental microbiology · 2008Article
- Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase.Applied and environmental microbiology · 2006Article
- Display of a thermostable lipase on the surface of a solvent-resistant bacterium, Pseudomonas putida GM730, and its applications in whole-cell biocatalysis.BMC biotechnology · 2006Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The strain Pseudomonas putida DOT-T1E was tested for its ability to tolerate second phases of different alkanols for their use as solvents in two-liquid-phase biotransformations. Although 1-decanol showed an about 10-fold higher toxicity to the cells than 1-octanol, the cells were able to adapt completely to 1-decanol only and could not be adapted in order to grow stably in the presence of a second phase of 1-octanol. The main explanation for this observation can be seen in the higher water and membrane solubility of 1-octanol. The hydrophobicity (log P) of a substance correlates with a certain partitioning of that compound into the membrane. Combining the log P value with the water solubility, the maximum membrane concentration of a compound can be calculated. With this simple calculation, it is possible to predict the property of an organic chemical for its potential applicability as a solvent for two-liquid-phase biotransformations with solvent-tolerant P. putida strains. Only compounds that show a maximum membrane concentration of less than 400 mM, such as 1-decanol, seem to be tolerated by these bacterial strains when applied in supersaturating concentrations to the medium. Taking into consideration that a solvent for a two-liquid-phase system should possess partitioning properties for potential substrates and products of a fine chemical synthesis, it can be seen that 1-decanol is a suitable solvent for such biotransformation processes. This was also demonstrated in shake cultures, where increasing amounts of a second phase of 1-decanol led to bacteria tolerating higher concentrations of the model substrate 3-nitrotoluene. Transferring this example to a 5-liter-scale bioreactor with 10% (vol/vol) 1-decanol, the amount of 3-nitrotoluene tolerated by the cells is up to 200-fold higher than in pure aqueous medium. The system demonstrates the usefulness of two-phase biotransformations utilizing solvent-tolerant bacteria.
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