ArticleApplied and environmental microbiology2005
Improvement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 127 citations in OpenAlex.
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- Label-free liquid chromatography-mass spectrometry comparison of the breast muscle proteome profiles in two fast-growing broilers.Scientific reports · 2024Article
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- Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae.Biotechnology letters · 2021Article
- Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- Quantitative Trait Nucleotides Impacting the Technological Performances of IndustrialFrontiers in genetics · 2019Article
- Multi-component gene network design as a survival strategy in diverse environments.BMC systems biology · 2018Article
- Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake.FEMS yeast research · 2018Article
- Repeated Cis-Regulatory Tuning of a Metabolic Bottleneck Gene during Evolution.Molecular biology and evolution · 2018Article
- A Mutation inApplied and environmental microbiology · 2018Article
- Reconstruction of thermotolerant yeast by one-point mutation identified through whole-genome analyses of adaptively-evolved strains.Scientific reports · 2016Article
- Biofuel Production Based on Carbohydrates from Both Brown and Red Macroalgae: Recent Developments in Key Biotechnologies.International journal of molecular sciences · 2016Review
- Enhanced leavening ability of baker's yeast by overexpression of SNR84 with PGM2 deletion.Journal of industrial microbiology & biotechnology · 2015Article
- Modeling Gene Networks in Saccharomyces cerevisiae Based on Gene Expression Profiles.Computational and mathematical methods in medicine · 2015Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Through genome-wide transcript analysis of a reference strain and two recombinant Saccharomyces cerevisiae strains with different rates of galactose uptake, we obtained information about the global transcriptional response to metabolic engineering of the GAL gene regulatory network. One of the recombinant strains overexpressed the gene encoding the transcriptional activator Gal4, and in the other strain the genes encoding Gal80, Gal6, and Mig1, which are negative regulators of the GAL system, were deleted. Even though the galactose uptake rates were significantly different in the three strains, we surprisingly did not find any significant changes in the expression of the genes encoding the enzymes catalyzing the first steps of the pathway (i.e., the genes encoding Gal2, Gal1, Gal7, and Gal10). We did, however, find that PGM2, encoding the major isoenzyme of phosphoglucomutase, was slightly up-regulated in the two recombinant strains with higher galactose uptake rates. This indicated that PGM2 is a target for overexpression in terms of increasing the flux through the Leloir pathway, and through overexpression of PGM2 the galactose uptake rate could be increased by 70% compared to that of the reference strain. Based on our findings, we concluded that phosphoglucomutase plays a key role in controlling the flux through the Leloir pathway, probably due to increased conversion of glucose-1-phosphate to glucose-6-phosphate. This conclusion was supported by measurements of sugar phosphates, which showed that there were increased concentrations of glucose-6-phosphate, galactose-6-phosphate, and fructose-6-phosphate in the strain construct overexpressing PGM2.
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