ArticleMicrobes and infection2005
Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus.
Article in Microbes and infection, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
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- Characterization of the cellular lipid composition during SARS-CoV-2 infection.Analytical and bioanalytical chemistry · 2023Article
- Choosing a cellular model to study SARS-CoV-2.Frontiers in cellular and infection microbiology · 2022Review
- Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.Journal of extracellular vesicles · 2021Article
- Pseudotyped Vesicular Stomatitis Virus-Severe Acute Respiratory Syndrome-Coronavirus-2 Spike for the Study of Variants, Vaccines, and Therapeutics Against Coronavirus Disease 2019.Frontiers in microbiology · 2021Review
- Article
- Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1.Clinical and vaccine immunology : CVI · 2011Article
- The 7a accessory protein of severe acute respiratory syndrome coronavirus acts as an RNA silencing suppressor.Journal of virology · 2010Article
- Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission.Journal of virology · 2010Review
- Persistent equine arteritis virus infection in HeLa cells.Journal of virology · 2008Article
- Persistent replication of severe acute respiratory syndrome coronavirus in human tubular kidney cells selects for adaptive mutations in the membrane protein.Journal of virology · 2008Article
- Cell type-specific cleavage of nucleocapsid protein by effector caspases during SARS coronavirus infection.Journal of molecular biology · 2008Article
- Enhancement of cytotoxicity against Vero E6 cells persistently infected with SARS-CoV by Mycoplasma fermentans.Archives of virology · 2007Article
- Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle.Microbes and infection · 2007Article
- Mechanisms of establishment of persistent SARS-CoV-infected cells.Biochemical and biophysical research communications · 2006Article
- Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV).Perspectives in medical virology · 2006Review
- Experimental Models for the Study of Central Nervous System Infection by SARS-CoV-2.Frontiers in immunologyReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little information is available on persistent infection of severe acute respiratory syndrome (SARS) coronavirus (CoV). In this study, we established persistent infection of SARS-CoV in the Vero E6 cell line. Acute infection of Vero E6 with SARS-CoV produced a lytic infection with characteristic rounding cytopathic effects (CPE) and the production of a large number of infectious particles in the culture fluid within 3 days post-infection. Upon subsequent culturing of the remaining adherent cells, the cells gradually proliferated and recovered normal morphology similar to that of the parental cells, and continued to produce large numbers of infectious viral particles during the observation period of 5 months. Among a total of 87 cell clones obtained from the persistently infected Vero E6, only four cell clones (named #13, #18, #21, and #34) were positive for viral RNA. Clones #13, #18, and #34 shifted to viral RNA-negative during subsequent cultures, while #21 continuously produced infectious particles at a high rate. The SARS-CoV receptor, angiotensin-converting enzyme 2, was almost completely down regulated from the cell surface of persistently infected cells. Western blot analysis as well as electron microscopy indicated that the ratios of spike to nucleocapsid protein in clone #21 as well as its parental persistently infected cells were lower than that in the cells in the acute phase of infection. These Vero E6 cells persistently infected with SARS-CoV may be useful for clarifying the mechanism of the persistent infection and also for elucidating the possible pathophysiologic significance of such long-term maintenance of this virus.
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