ArticleJournal of clinical periodontology2005
Nicotine inhibits human gingival fibroblast migration via modulation of Rac signalling pathways.
Article in Journal of clinical periodontology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Prevalence of smokeless tobacco use and its impact on periodontal health among adults in Rajnandgaon, Chhattisgarh, India: a cross-sectional study.Frontiers in oral health · 2025Article
- Cigarette smoking exposure disrupts the regenerative potential of dental pulp stem cells.Tobacco induced diseases · 2023Article
- Influence of inflammasome NLRP3, and IL1B and IL2 gene polymorphisms in periodontitis susceptibility.PloS one · 2020Article
- Review
- Antioxidant role of melatonin against nicotine's teratogenic effects on embryonic bone development.Iranian journal of basic medical sciences · 2018Article
- α7-nAChR Activation Has an Opposite Effect on Healing of Covered and Uncovered Wounds.Inflammation · 2018Article
- Exploration of the wound healing effect of topical administration of nicotine in combination with collagen scaffold in a rabbit model.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2016Article
- Cytotoxicity and apoptosis induction by e-cigarette fluids in human gingival fibroblasts.Clinical oral investigations · 2016Article
- Impact of high glucose on metastasis of colon cancer cells.World journal of gastroenterology · 2015Article
- Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.American journal of physiology. Endocrinology and metabolism · 2014Article
- Coronally Positioned Flap for Root Coverage: Comparison between Smokers and Nonsmokers.Journal of international oral health : JIOH · 2013Review
- Contribution of orthodontic load to inflammation-mediated periodontal destruction.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2010Article
- Sub-epithelial connective tissue graft for root coverage in nonsmokers and smokers: A pilot comparative clinical study.Journal of Indian Society of PeriodontologyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
aimCigarette smoking is a risk factor in the development of periodontal diseases. In addition, a delayed healing process has been shown in smokers compared with non-smokers after periodontal treatment. Cell migration is a key process of wound healing and it is highly regulated by a variety of signalling pathways. The small G protein, Rac, is necessary for cell migration. Our aim was to determine if nicotine disrupted Rac and its downstream signalling proteins, p21-activated kinase 1/2 (PAK1/2), and p44/42 mitogen-activated protein kinase (MAPK) (extracellular regulated kinase 1/2). MATERIAL AND
methodsPrimary human fibroblasts from healthy gingival tissues were cultured and grown to confluence. Cells were serum starved for 24 h, and then treated with nicotine (0 or 0.5 microM) prior to in vitro wounding. Cell migration was analysed in live cell assays following in vitro wounds. Rac activity, phosphorylation levels of PAK1/2, and p44/42 MAPK were assessed in cultures treated with or without nicotine after multiple wounds.
resultsNicotine decreased cell migration rates by 50% compared with controls. In addition, nicotine altered the activation patterns of Rac and PAK 1/2 and up-regulated p44/42 MAPK.
conclusionDecreased cell migration in periodontal wounds exposed to nicotine may be mediated through the Rac and PAK1/2 signalling pathways.
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