Evidence map›Paper›PMID 16267675›Full record

ArticleChromosoma2006

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy.

Elizabeth Schroeder-Reiter, Andreas Houben, Jürke Grau, Gerhard Wanner

Abstract read
PubMed Publisher
In one paragraph

Article in Chromosoma, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 14 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Elizabeth Schroeder-ReiterDepartment of Biology I, Ludwig-Maximillians-Universität München, Germany.
Andreas Houben
Jürke Grau
Gerhard Wanner
Ludwig-Maximilians-Universität München · DEBayerische Staatssammlung für Paläontologie und Geologie · DELeibniz Institute of Plant Genetics and Crop Plant Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An atypical peg-like terminal constriction ("peg") on metaphase chromosomes of the plant genus Oziroë could be identified as a nucleolus organizing region (NOR) by detecting 45S rDNA with correlative light microscopy (LM) and scanning electron microscopy (SEM) in situ hybridization (ISH). Using high-resolution 3D analytical SEM, the architecture and DNA distribution of the peg-like NOR were characterized as typical for chromosomes, albeit with significantly smaller chromomeres. ISH procedure was improved for SEM concerning signal localization, labeling efficiency, and structural preservation, allowing 3D SEM analysis of the peg-like NOR structure and rDNA distribution for the first time. It could be shown that implementation of FluoroNanogold markers is an attractive tool that allows efficient immunodection in both LM and SEM. A model is proposed for the peg structure and its mode of condensation.

Indexed as

Cell CycleDNA, PlantDNA, RibosomalIn Situ HybridizationLiliaceaeMicroscopyMicroscopy, Electron, ScanningNucleolus Organizer RegionDNA, PlantDNA, Ribosomal

Identifiers

PMID16267675
OpenAlexW2019551311

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.