ArticleJournal of bacteriology2005
Diverse phenotypes resulting from polyphosphate kinase gene (ppk1) inactivation in different strains of Helicobacter pylori.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Isolation and characterization of a potential heterotrophic nitrifying-aerobic denitrifying and phosphorus-accumulating bacteriumWater science and technology : a journal of the International Association on Water Pollution Research · 2026Article
- Inorganic Polyphosphate: An Emerging Regulator of Neuronal Bioenergetics and Its Implications in Neuroprotection.Biomolecules · 2025Review
- An Update on Polyphosphate In Vivo Activities.Biomolecules · 2024Review
- Functional characterization of the gonococcal polyphosphate pseudo-capsule.PLoS pathogens · 2023Article
- Article
- Bacterial Defense Systems against the Neutrophilic Oxidant Hypochlorous Acid.Infection and immunity · 2020Review
- Inorganic polyphosphate in mammals: where's Wally?Biochemical Society transactions · 2020Review
- Environmental phosphate differentially affects virulence phenotypes of uropathogenic Escherichia coli isolates causative of prostatitis.Virulence · 2015Article
- Phenotypic screening of a targeted mutant library reveals Campylobacter jejuni defenses against oxidative stress.Infection and immunity · 2014Article
- Functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) of Campylobacter jejuni.Virulence · 2014Article
- Polyphosphate degradation in stationary phase triggers biofilm formation via LuxS quorum sensing system in Escherichia coli.PloS one · 2012Article
- Comparative transcriptomics of H. pylori strains AM5, SS1 and their hpyAVIBM deletion mutants: possible roles of cytosine methylation.PloS one · 2012Article
- Polyphosphate kinase 2: a novel determinant of stress responses and pathogenesis in Campylobacter jejuni.PloS one · 2010Article
- Polyphosphate binds to the principal sigma factor of RNA polymerase during starvation response in Helicobacter pylori.Molecular microbiology · 2010Article
- ppGpp conjures bacterial virulence.Microbiology and molecular biology reviews : MMBR · 2010Review
- Importance of polyphosphate kinase 1 for Campylobacter jejuni viable-but-nonculturable cell formation, natural transformation, and antimicrobial resistance.Applied and environmental microbiology · 2009Article
- Helicobacter pylori HopQ outer membrane protein attenuates bacterial adherence to gastric epithelial cells.FEMS microbiology letters · 2008Article
- Identification of Campylobacter jejuni genes involved in the response to acidic pH and stomach transit.Applied and environmental microbiology · 2008Article
- Expanding the Helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector.Applied and environmental microbiology · 2007Article
- Polyphosphate kinase 1 is a pathogenesis determinant in Campylobacter jejuni.Journal of bacteriology · 2007Article
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6 authors.
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Abstract
Connections among biochemical pathways should help buffer organisms against environmental stress and affect the pace and trajectory of genome evolution. To explore these ideas, we studied consequences of inactivating the gene for polyphosphate kinase 1 (ppk1) in strains of Helicobacter pylori, a genetically diverse gastric pathogen. The PPK1 enzyme catalyzes synthesis of inorganic polyphosphate (poly P), a reservoir of high-energy phosphate bonds with multiple roles. Prior analyses in less-fastidious microbes had implicated poly P in stress resistance, motility, and virulence. In our studies, ppk1 inactivation caused the expected near-complete absence of poly P (>250-fold decrease) but had phenotypic effects that differed markedly among unrelated strains: (i) poor initial growth on standard brain heart infusion agar (five of six strains tested); (ii) weakened colonization of mice (4 of 5 strains); (iii) reduced growth on Ham's F-12 agar, a nutritionally limiting medium (8 of 11 strains); (iv) heightened susceptibility to metronidazole (6 of 17 strains); and (v) decreased motility in soft agar (1 of 13 strains). Complementation tests confirmed that the lack of growth of one Deltappk1 strain on F-12 agar and the inability to colonize mice of another were each due to ppk1 inactivation. Thus, the importance of ppk1 to H. pylori differed among strains and the phenotypes monitored. We suggest that quantitative interactions, as seen here, are common among genes that affect metabolic pathways and that H. pylori's high genetic diversity makes it well suited for studies of such interactions, their underlying mechanisms, and their evolutionary consequences.
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