ArticleJournal of bacteriology2005
Adaptations of Pseudomonas aeruginosa to the cystic fibrosis lung environment can include deregulation of zwf, encoding glucose-6-phosphate dehydrogenase.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Comparison of clinical and microbiological features between mucoid and non-mucoidMicrobiology spectrum · 2026Article
- Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches.Microbiology spectrum · 2023Article
- Sanguinarine Inhibits the 2-Ketogluconate Pathway of Glucose Utilization inFrontiers in microbiology · 2021Article
- The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon.Scientific reports · 2018Article
- Control of primary metabolism by a virulence regulatory network promotes robustness in a plant pathogen.Nature communications · 2018Article
- Pseudomonas aeruginosa and Klebsiella pneumoniae Adaptation to Innate Immune Clearance Mechanisms in the Lung.Journal of innate immunity · 2018Review
- Mutation-Driven Divergence and Convergence Indicate Adaptive Evolution of the Intracellular Human-Restricted Pathogen, Bartonella bacilliformis.PLoS neglected tropical diseases · 2016Article
- Intraclonal genome diversity of the major Pseudomonas aeruginosa clones C and PA14.Environmental microbiology reports · 2016Article
- Metabolic crosstalk between host and pathogen: sensing, adapting and competing.Nature reviews. Microbiology · 2016Review
- New insights into TB physiology suggest untapped therapeutic opportunities.Immunological reviews · 2015Review
- Fosfomycin and tobramycin in combination downregulate nitrate reductase genes narG and narH, resulting in increased activity against Pseudomonas aeruginosa under anaerobic conditions.Antimicrobial agents and chemotherapy · 2013Article
- Bioinformatic prediction of gene functions regulated by quorum sensing in the bioleaching bacterium Acidithiobacillus ferrooxidans.International journal of molecular sciences · 2013Article
- Metabolite profiling to characterize disease-related bacteria: gluconate excretion by Pseudomonas aeruginosa mutants and clinical isolates from cystic fibrosis patients.The Journal of biological chemistry · 2013Article
- Detecting bacterial lung infections: in vivo evaluation of in vitro volatile fingerprints.Journal of breath research · 2013Article
- Fosfomycin enhances the active transport of tobramycin in Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2012Article
- Clinical significance of microbial infection and adaptation in cystic fibrosis.Clinical microbiology reviews · 2011Review
- The genetic basis of laboratory adaptation in Caulobacter crescentus.Journal of bacteriology · 2010Article
- Nutrient availability as a mechanism for selection of antibiotic tolerant Pseudomonas aeruginosa within the CF airway.PLoS pathogens · 2010Article
- Isocitrate lyase supplies precursors for hydrogen cyanide production in a cystic fibrosis isolate of Pseudomonas aeruginosa.Journal of bacteriology · 2009Article
- Differential expression of Haemophilus parasuis genes in response to iron restriction and cerebrospinal fluid.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2007Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginate production in the mucoid, CF isolate, P. aeruginosa FRD1. The main regulator of alginate, sigma-22 encoded by algT (algU), plays a small but demonstrable role in the induction of zwf expression in P. aeruginosa. However, G6PDH activity and zwf expression were higher in FRD1 strains than in PAO1 strains. In PAO1, zwf expression and G6PDH activity are known to be subject to catabolite repression by succinate. In contrast, FRD1 zwf expression and G6PDH activity were shown to be refractory to such catabolite repression. This was apparently not due to a defect in the catabolite repression control (Crc) protein. Such relaxed control of zwf was found to be common among several examined CF isolates but was not seen in other strains of clinical and environmental origin. Two sets of clonal isolates from individual CF patient indicated that the resident P. aeruginosa strain underwent an adaptive change that deregulated zwf expression. We hypothesized that high-level, unregulated G6PDH activity provided a survival advantage to P. aeruginosa within the lung environment. Interestingly, zwf expression in P. aeruginosa was shown to be required for its resistance to human sputum. This study illustrates that adaptation to the CF pulmonary environment by P. aeruginosa can include altered regulation of basic metabolic activities, including carbon catabolism.
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