ArticleProceedings of the National Academy of Sciences of the United States of America2005
Biofilm formation and phenotypic variation enhance predation-driven persistence of Vibrio cholerae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 159 papers.
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Who cites it
159 citing papers in PubMed.
- Enhanced Endophytic Colonization of Flavobacterium Promotes Plant Resistance to Fusarium Wilt.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- H-NOX and NosP Regulate Flagellar Protein and Virulence Factor Production in Vibrio Cholerae.Molecular & cellular proteomics : MCP · 2026Article
- Predation in microbial communities: gradients of nutritive killing.Nature reviews. Microbiology · 2026Review
- Preliminary Insights into the Seasonal Variation, Phylogenetic Diversity, and Biofilm-Forming Capacity of CultivableMicroorganisms · 2026Article
- A branching cell-fate decision in biofilm dispersal enables long-term surface persistence.bioRxiv : the preprint server for biology · 2026Article
- Predator-prey dynamics of Vibrio cholerae on chitin suggest an alternative mode of biofilm formation in marine snow conditions.The ISME journal · 2026Article
- The Role of Biofilm-Derived Compounds in Microbial and Protozoan Interactions.Microorganisms · 2025Review
- A small periplasmic protein governs broad physiological adaptations in Vibrio cholerae via regulation of the DbfRS two-component system.Nature communications · 2025Article
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- Parallel regulatory circuits orchestrate biofilm formation in response to c-di-GMP levels and growth phase.PLoS genetics · 2025Article
- Enigmatic roles ofJournal of bacteriology · 2025Review
- Quorum quenching of phenolic compounds from Leptolyngbya spp. MACC 32 via downregulation of the periplasmic receptor LuxP in Vibrio harveyi: Hinting a putative mechanism.World journal of microbiology & biotechnology · 2025Article
- Comprehensive genomic and evolutionary analysis of biofilm matrix clusters and proteins in themSystems · 2025Article
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- Screening and functional characterization of isocitrate lyase AceA in the biofilm formation ofApplied and environmental microbiology · 2024Article
- Biofilm dispersal patterns revealed using far-red fluorogenic probes.PLoS biology · 2024Article
- Dispersal of a dominant competitor can drive multispecies coexistence in biofilms.Current biology : CB · 2024Article
- Review
- Biofilm dispersal patterns revealed using far-red fluorogenic probes.bioRxiv : the preprint server for biology · 2024Article
99 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Persistence of the opportunistic bacterial pathogen Vibrio cholerae in aquatic environments is the principal cause for seasonal occurrence of cholera epidemics. This causality has been explained by postulating that V. cholerae forms biofilms in association with animate and inanimate surfaces. Alternatively, it has been proposed that bacterial pathogens are an integral part of the natural microbial food web and thus their survival is constrained by protozoan predation. Here, we report that both explanations are interrelated. Our data show that biofilms are the protective agent enabling V. cholerae to survive protozoan grazing while their planktonic counterparts are eliminated. Grazing on planktonic V. cholerae was found to select for the biofilm-enhancing rugose phase variant, which is adapted to the surface-associated niche by the production of exopolymers. Interestingly, grazing resistance in V. cholerae biofilms was not attained by exopolymer production alone but was accomplished by the secretion of an antiprotozoal factor that inhibits protozoan feeding activity. We identified that the cell density-dependent regulator hapR controls the production of this factor in biofilms. The inhibitory effect of V. cholerae biofilms was found to be widespread among toxigenic and nontoxigenic isolates. Our results provide a mechanistic explanation for the adaptive advantage of surface-associated growth in the environmental persistence of V. cholerae and suggest an important contribution of protozoan predation in the selective enrichment of biofilm-forming strains in the out-of-host environment.
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