ArticleThe Journal of biological chemistry2006
Two isoforms of a divalent metal transporter (DMT1) in Schistosoma mansoni suggest a surface-associated pathway for iron absorption in schistosomes.
Article in The Journal of biological chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Antimicrobial Peptides (AMPs): Potential Therapeutic Strategy against Trypanosomiases?Biomolecules · 2023Review
- A new ferritin SjFer0 affecting the growth and development of Schistosoma japonicum.Parasites & vectors · 2022Article
- Orchestrating a heist: uptake and storage of metals by apicomplexan parasites.Microbiology (Reading, England) · 2021Article
- Transcriptome of the parasitic flatworm Schistosoma mansoni during intra-mammalian development.PLoS neglected tropical diseases · 2020Article
- Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.International journal for parasitology. Drugs and drug resistance · 2018Article
- Interactions and accumulation differences of metal(loid)s in three sea cucumber species collected from the Northern Mediterranean Sea.Environmental science and pollution research international · 2016Article
- Schistosome feeding and regurgitation.PLoS pathogens · 2014Review
- Antischistosomal activity of trioxaquines: in vivo efficacy and mechanism of action on Schistosoma mansoni.PLoS neglected tropical diseases · 2012Article
- Characterization of the phytochelatin synthase of Schistosoma mansoni.PLoS neglected tropical diseases · 2011Article
- A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum.PLoS neglected tropical diseases · 2010Article
- Pumping iron: a potential target for novel therapeutics against schistosomes.Trends in parasitology · 2007Review
- Tracking the fate of iron in early development of human blood flukes.The international journal of biochemistry & cell biology · 2007Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
We describe two homologues of the mammalian divalent metal transporter (DMT1) for Schistosoma mansoni, a pathogenic intravascular parasite of humans. Schistosomes have a high nutritional and metabolic demand for iron. Nucleotide sequences of the parasite homologues, designated SmDMT1A and -B, are identical in all but the 5'-regions. The predicted amino acid sequences share at least 60% identity with DMT1 (=Nramp2) of humans, mice, and rats, and at least 55% identity with Nramp1 from mice, humans and Caenorhabditis elegans. SmDMT1A is expressed in differentiating eggs, miracidia, cercariae, schistosomula, and adults, whereas SmDMT1B is expressed in all but the miracidium and occurs at lower levels than SmDMT1A in differentiating eggs and cercariae. An iron-responsive element, present at the 3'-untranslated region of many DMT1 molecules, is not present in schistosome mRNAs studied here. A Western blot analysis of adult worm preparations using a homologous rabbit serum raised against a schistosome DMT1 peptide and a heterologous serum raised against mammalian DMT1, revealed a band approximating 115 kDa. By immunofluorescence microscopy, the schistosome DMT1s localize primarily to the tegument. Iron uptake assays demonstrated that SmDMT1s were able to rescue yeast growth in ferrous iron-transport deficient yeast (fet3fet4). The results suggest that schistosomes express molecules for ferrous iron transport in their tegument, suggesting trans-tegumental transport as one means of iron acquisition for these parasites.
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