ArticleProceedings of the National Academy of Sciences of the United States of America2005
Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
113 citing papers in PubMed, 360 citations in OpenAlex.
- Biased retention and functional diversification of CESA superfamily genes in Medicago mediating development and abiotic stress responses.BMC plant biology · 2026Article
- Integrated analysis of transcriptome and metabolome reveals the mechanism of lignin biosynthesis in fruit abscission of Chinese bayberry (Myrica rubra).BMC plant biology · 2025Article
- Identification of Quality-Related Genomic Regions and Candidate Genes in Silage Maize by Combining GWAS and Meta-Analysis.Plants (Basel, Switzerland) · 2025Article
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Abiotic stress responses in forage crops and grasses: the role of secondary metabolites and biotechnological interventions.Frontiers in plant science · 2025Review
- Transcriptome analysis reveals changes in lignin and flavonoid biosynthesis inFrontiers in plant science · 2025Article
- Nutritional enhancement of animal feed and forage crops via genetic modification.Journal of the Royal Society of New Zealand · 2025Review
- Integrated Multi-Omics Analysis to Reveal the Molecular Mechanisms of Inflorescence Elongation inInternational journal of molecular sciences · 2024Article
- The Integration of Transcriptome and Metabolome Analyses Provides Insights into the Determinants of the Wood Properties inInternational journal of molecular sciences · 2024Article
- Alfalfa Responses to Intensive Soil Compaction: Effects on Plant and Root Growth, Phytohormones and Internal Gene Expression.Plants (Basel, Switzerland) · 2024Article
- Mutating alfalfaFrontiers in plant science · 2024Article
- Article
- Multi-trait modeling and machine learning discover new markers associated with stem traits in alfalfa.Frontiers in plant science · 2024Article
- Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Lignin, the Lignification Process, and Advanced, Lignin-Based Materials.International journal of molecular sciences · 2023Review
- Systematic approaches to C-lignin engineering in Medicago truncatula.Biotechnology for biofuels and bioproducts · 2023Article
- Evaluation of Rumen Degradation Kinetics of Low-Lignin Alfalfa 'Hi-GestAnimals : an open access journal from MDPI · 2023Article
- Overexpression of PtoMYB115 improves lignocellulose recalcitrance to enhance biomass digestibility and bioethanol yield by specifically regulating lignin biosynthesis in transgenic poplar.Biotechnology for biofuels and bioproducts · 2022Article
- Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes.Plants (Basel, Switzerland) · 2022Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improving the digestibility of forages provides a means to enhance animal performance and protect the environment against excessive animal waste. Increased lignin content during maturity, and corresponding changes in lignin composition, correlate with decreased digestibility of forages. These relationships have yet to be investigated in isogenic systems. By targeting three specific cytochrome P450 enzymes of the lignin pathway for antisense down-regulation, we generated transgenic alfalfa lines with a range of differences in lignin content and composition. There was a strong negative relationship between lignin content and rumen digestibility, but no relationship between lignin composition and digestibility, in these transgenic lines. Models for genetic manipulation of forage digestibility based on the changes in lignin composition that increase paper-pulping efficiency in trees are therefore invalid. Down-regulation of 4-coumarate 3-hydroxylase provided the largest improvements in digestibility yet seen in a forage crop.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.