ArticleProceedings of the National Academy of Sciences of the United States of America2005
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 260 papers, 1 of them a synthesis that pooled it.
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Who cites it
260 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Imaging and quantifying drug delivery in skin - Part 2: Fluorescence andvibrational spectroscopic imaging methods.Advanced drug delivery reviews · 2020Pooled it
- Shining a light on brain tumors: a review of Raman spectroscopy in the operating room.Journal of biomedical optics · 2026Review
- Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy.Science advances · 2026Article
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Five-modal multiphoton microscopy enabled by a tunable dual-wavelength fiber laser.Biomedical optics express · 2026Article
- Ultrastructural analysis of human uncinate fasciculus with coherent anti-Stokes Raman spectroscopy.Biophotonics discovery · 2026Article
- Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.Analytical chemistry · 2026Article
- Advancing Label-Free Imaging Through CARS Microscopy: From Signal Formation to Biological Interpretation.International journal of molecular sciences · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Rapid hyperspectral Raman imaging of cells with depthwise separable 3D MultiResU-Net.iScience · 2025Article
- Article
- Nonlinear optical microscopy with an obscuration-free, freeform reflective objective.Biomedical optics express · 2025Article
- Interface-Sensitive Epi-Coherent Anti-Stokes Raman Scattering Microscopy for Imaging Cell Adhesion Dynamics.bioRxiv : the preprint server for biology · 2025Article
- Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.Nature communications · 2025Article
- Lipid droplet accumulation in microglia and their potential roles.Lipids in health and disease · 2025Review
- Raman spectroscopy and imaging of protein droplet formation and aggregation.Current opinion in structural biology · 2025Review
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Broadband CARS Hyperspectral Classification of Single Immune Cells.Journal of biophotonics · 2025Article
- Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.PloS one · 2025Article
- Unveiling the Molecular Secrets: A Comprehensive Review of Raman Spectroscopy in Biological Research.ACS omega · 2024Review
200 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with molecular specificity. We have developed a sensitive technique for vibrational imaging of tissues by combining coherent anti-Stokes Raman scattering (CARS) with video-rate microscopy. Backscattering of the intense forward-propagating CARS radiation in tissue gives rise to a strong epi-CARS signal that makes in vivo imaging possible. This substantially large signal allows for real-time monitoring of dynamic processes, such as the diffusion of chemical compounds, in tissues. By tuning into the CH(2) stretching vibrational band, we demonstrate CARS imaging and spectroscopy of lipid-rich tissue structures in the skin of a live mouse, including sebaceous glands, corneocytes, and adipocytes, with unprecedented contrast at subcellular resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.