ArticleNeurochemical research2005
Ceramide induces non-apoptotic cell death in human glioma cells.
Article in Neurochemical research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer.Nature communications · 2020Article
- The development of the concept of ferroptosis.Free radical biology & medicine · 2019Review
- Comparison of a ceramidase inhibitor (ceranib-2) with C2 ceramide and cisplatin on cytotoxicity and apoptosis of glioma cells.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- The role of necroptosis in neurosurgical diseases.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Review
- Quercetin promotes proliferation and differentiation of oligodendrocyte precursor cells after oxygen/glucose deprivation-induced injury.Cellular and molecular neurobiology · 2014Article
- Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death.Biochimica et biophysica acta · 2013Article
- Neutral sphingomyelinase 2 modulates cytotoxic effects of protopanaxadiol on different human cancer cells.BMC complementary and alternative medicine · 2013Article
- Programmed necrosis: backup to and competitor with apoptosis in the immune system.Nature immunology · 2011Review
- Molecular mechanisms of necroptosis: an ordered cellular explosion.Nature reviews. Molecular cell biology · 2010Review
- The pivotal role of c-Jun NH2-terminal kinase-mediated Beclin 1 expression during anticancer agents-induced autophagy in cancer cells.Oncogene · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ceramide causes either apoptosis or non-apoptotic cell death depending on model system and experimental conditions. The present study was undertaken to examine the effect of ceramide on cell viability and its molecular events leading to cell death in A172 human glioma cells. Ceramide induced cell death in a dose-dependent manner and the cell death was dependent on generation of reactive oxygen species and lipid peroxidation. TUNEL assay, Hoechst 33258 staining, and flow cytometric analysis did not show typical apoptotic morphological features. Ceramide caused phosphorylation of extracellular signal-regulated kinase (ERK) and p38, but the cell death was not affected by inhibitors of MAPK subfamilies. Ceramide caused ATP depletion without loss of mitochondrial membrane potential. Ceramide did not induce caspase activation and ceramide-induced cell death was also not altered by inhibitors of caspase activation. Transfection of dominant inhibitory mutant of IkappaBalpha (S32A/36A) and pretreatment of pyrrolidinedithiocarbamate, an inhibitor of NF-kappaB, enhanced ceramide-induced cell death. These results indicate that ceramide causes non-apoptotic, caspase-independent cell death by inducing reactive oxygen species generation in A172 human glioma cells. NF-kappaB is involved in the regulation of ceramide-induced cell death in human glioma cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.