ArticleThe Journal of molecular diagnostics : JMD2005
Molecular diagnosis of necrotizing fasciitis by 16S rRNA gene sequencing and superantigen gene detection.
Article in The Journal of molecular diagnostics : JMD, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- High-throughput 16S rDNA sequencing assisting in the detection of bacterial pathogen candidates: a fatal case of necrotizing fasciitis in a child.International journal of legal medicine · 2021Article
- Clinically Important Toxins in Bacterial Infection: Utility of Laboratory Detection.Clinical microbiology newsletter · 2020Article
- Necrotizing fasciitis: case series and review of the literature on clinical and medico-legal diagnostic challenges.International journal of legal medicine · 2018Review
- An efficient alternative marker for specific identification of Mycobacterium tuberculosis.World journal of microbiology & biotechnology · 2014Article
- Soft tissue infections caused by marine bacterial pathogens: epidemiology, diagnosis, and management.Current infectious disease reports · 2011Article
- Growth of adenocarcinoma on routine microbiological media inoculated with fluid from a pleural effusion in an 82-year-old female.Journal of clinical microbiology · 2009Article
- Necrotizing fasciitis from Vibrio vulnificus in a patient with undiagnosed hepatitis and cirrhosis.Journal of clinical microbiology · 2007Article
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Authors and funding
4 authors.
Funding
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Abstract
We report the use of molecular techniques in the diagnosis of a case of culture-negative necrotizing fasciitis occurring in a 32-year-old female with no significant past medical history and who died within 36 hours of admission. Paraffin-embedded tissue sections from the popliteal fossa region obtained at autopsy showed hemorrhage, necrosis, and mild inflammation by hematoxylin and eosin staining. Tissue gram stain showed numerous gram-positive organisms arranged in clusters. The sequences of the first 500 bp of bacterial 16S rRNA gene amplified from the lesion were identical to a Lancefield group A beta-hemolytic Streptococcus pyogenes. Streptococcal pyrogenic exotoxin A and B superantigen genes were detected and an emm type 1 was determined by polymerase chain reaction and sequencing from the lesion. This confirmed the etiology of the patient's rapid deterioration with multisystem organ failure.
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